MicroRNA-mediated regulation of pancreatic cancer cell proliferation

  • Authors:
    • Aruna Basu
    • Xin Jiang
    • Massimo Negrini
    • Subrata Haldar
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  • Published online on: May 1, 2010     https://doi.org/10.3892/ol_00000100
  • Pages: 565-568
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Abstract

MicroRNAs (miRNAs) comprising 19-25 nucleotides are highly conserved small non-coding RNAs which regulate normal gene expression during development, cell proliferation and apoptosis by targeting mRNAs of protein-coding genes at the post-transcriptional level. Prevalent studies suggest that some human miRNAs, such as miRNA-16, are deregulated in human cancer and behave as tumor suppressors. The overall objective of our investigation was to assess whether miRNA-16 (miR-16) is involved in the regulation of critical genes, such as BCL2, that control the sensitivity of pancreatic cancer cells to apoptosis. This study showed that the ectopic overexpression of miR-16 may be therapeutically beneficial as is evidenced by impaired cell survival with concomitant attenuation of anti-apoptotic protein Bcl-2. Moreover, the luciferase reporter assay suggested that miR-16 post-transcriptionally regulates Bcl-2 expression in pancreatic cancer cells through the target sites of the 3' untranslated region of this gene.
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May-June 2010
Volume 1 Issue 3

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Basu A, Jiang X, Negrini M and Haldar S: MicroRNA-mediated regulation of pancreatic cancer cell proliferation . Oncol Lett 1: 565-568, 2010
APA
Basu, A., Jiang, X., Negrini, M., & Haldar, S. (2010). MicroRNA-mediated regulation of pancreatic cancer cell proliferation . Oncology Letters, 1, 565-568. https://doi.org/10.3892/ol_00000100
MLA
Basu, A., Jiang, X., Negrini, M., Haldar, S."MicroRNA-mediated regulation of pancreatic cancer cell proliferation ". Oncology Letters 1.3 (2010): 565-568.
Chicago
Basu, A., Jiang, X., Negrini, M., Haldar, S."MicroRNA-mediated regulation of pancreatic cancer cell proliferation ". Oncology Letters 1, no. 3 (2010): 565-568. https://doi.org/10.3892/ol_00000100