Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of breast cancer cell line MDA-MB-231

  • Authors:
    • Yonghong Shi
    • Miaomiao Chang
    • Fang Wang
    • Xiaohui Ouyang
    • Yongfeng Jia
    • Hua Du
  • View Affiliations

  • Published online on: July 1, 2010     https://doi.org/10.3892/ol_00000115
  • Pages: 657-662
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Abstract

The role of hypoxia-inducible factor-1 (HIF-1) in tumor development and progression is well-established but its effect on tumor growth remains controversial. The present study investigated the effect of HIF-1 on tumor growth using the estrogen receptor-negative breast cancer cell line, MDA-MB-231. Using Western blotting, we detected a higher level of HIF-1α protein in MDA-MB-231 cells than in any other breast cancer cell lines analyzed, and this was accompanied by a more rapid growth pattern. Interruption of HIF-1α expression using small interference RNA (siRNA) significantly suppressed cell growth and increased apoptosis, but the cell cycle was not affected. Activated fragments with increased caspase 3 activity and a mobility shift of B cell lymphoma (Bcl-2) were also detected in cells treated with HIF-1α siRNA. HIF-1 allows breast cancer cells to grow under long-term serum deprivation by inactivation of the caspase cascade and thus inhibition of apoptosis. Blocking HIF-1α protein resulted in loss of Bcl-2 function, which may contribute to the activation of the caspase cascade.
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July-August 2010
Volume 1 Issue 4

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Shi Y, Chang M, Wang F, Ouyang X, Jia Y and Du H: Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of breast cancer cell line MDA-MB-231 . Oncol Lett 1: 657-662, 2010
APA
Shi, Y., Chang, M., Wang, F., Ouyang, X., Jia, Y., & Du, H. (2010). Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of breast cancer cell line MDA-MB-231 . Oncology Letters, 1, 657-662. https://doi.org/10.3892/ol_00000115
MLA
Shi, Y., Chang, M., Wang, F., Ouyang, X., Jia, Y., Du, H."Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of breast cancer cell line MDA-MB-231 ". Oncology Letters 1.4 (2010): 657-662.
Chicago
Shi, Y., Chang, M., Wang, F., Ouyang, X., Jia, Y., Du, H."Role and mechanism of hypoxia-inducible factor-1 in cell growth and apoptosis of breast cancer cell line MDA-MB-231 ". Oncology Letters 1, no. 4 (2010): 657-662. https://doi.org/10.3892/ol_00000115