Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Oncology Letters
      • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Biomedical Reports
      • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • Information for Authors
    • Information for Reviewers
    • Information for Librarians
    • Information for Advertisers
    • Conferences
  • Language Editing
Spandidos Publications Logo
  • About
    • About Spandidos
    • Aims and Scopes
    • Abstracting and Indexing
    • Editorial Policies
    • Reprints and Permissions
    • Job Opportunities
    • Terms and Conditions
    • Contact
  • Journals
    • All Journals
    • Biomedical Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Experimental and Therapeutic Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Epigenetics
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Functional Nutrition
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Molecular Medicine
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • International Journal of Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Medicine International
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular and Clinical Oncology
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Molecular Medicine Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Letters
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • Oncology Reports
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
    • World Academy of Sciences Journal
      • Information for Authors
      • Editorial Policies
      • Editorial Board
      • Aims and Scope
      • Abstracting and Indexing
      • Bibliographic Information
      • Archive
  • Articles
  • Information
    • For Authors
    • For Reviewers
    • For Librarians
    • For Advertisers
    • Conferences
  • Language Editing
Login Register Submit
  • This site uses cookies
  • You can change your cookie settings at any time by following the instructions in our Cookie Policy. To find out more, you may read our Privacy Policy.

    I agree
Search articles by DOI, keyword, author or affiliation
Search
Advanced Search
presentation
Oncology Letters
Join Editorial Board Propose a Special Issue
Print ISSN: 1792-1074 Online ISSN: 1792-1082
Journal Cover
July-August 2010 Volume 1 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

Journal Cover
July-August 2010 Volume 1 Issue 4

Full Size Image

Sign up for eToc alerts
Recommend to Library

  • Article
  • Citations
    • Cite This Article
    • Download Citation
    • Create Citation Alert
    • Remove Citation Alert
    • Cited By
  • Similar Articles
    • Related Articles (in Spandidos Publications)
    • Similar Articles (Google Scholar)
    • Similar Articles (PubMed)
  • Download PDF
  • Download XML
  • View XML
Article

Detection of P53 mutations in different cancer types is improved by cDNA sequencing

  • Authors:
    • Sylwester Piaskowski
    • Izabela Zawlik
    • Malgorzata Szybka
    • Dominika Kulczycka-Wojdala
    • Ewelina Stoczynska-Fidelus
    • Michal Bienkowski
    • Tadeusz Robak
    • Renata Kusinska
    • Dorota Jesionek-Kupnicka
    • Radzislaw Kordek
    • Piotr Rieske
    • Pawel P. Liberski
  • View Affiliations / Copyright

    Affiliations: Department of Molecular Pathology and Neuropathology, Chair of Oncology, Medical University of Lodz, 92-216 Lodz, Poland
  • Pages: 717-721
    |
    Published online on: July 1, 2010
       https://doi.org/10.3892/ol_00000125
  • Expand metrics +
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Metrics: Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )
Cited By (CrossRef): 0 citations Loading Articles...

This article is mentioned in:



Abstract

Recently published data show discrepancies between P53 cDNA and DNA sequencing results in glioblastoma, colorectal cancer and pleomorphic xanthoastrocytoma. We hypothesized that similar discrepancies are observed in other types of human cancers. Using DNA and cDNA direct sequencing, we analyzed 40 cases of invasive breast duct carcinoma, 23 cases of acute myeloblastic leukaemia, 12 cases of astrocytoma and 40 cases of soft tissue sarcoma for P53 mutations. Additionally, we used real-time quantitative PCR to estimate the normalized relative P53 expression. In the comparative study, the P53 mutation was detected more frequently when using cDNA sequencing than DNA sequencing in all of the cancer types. Furthermore, several samples presented missense P53 mutations, with visible wild-type nucleotide on the DNA sequence. In contrast, elimination of the wild-type allele or selective overproduction of the mutated allele was observed on the cDNA sequence. P53 expression was not significantly different between the cases with or without P53 mutations. These results indicate that cDNA sequencing improves the detection of P53 mutations in these cancers. We suggest that the true incidence of P53 mutations in these cancers is underestimated at the DNA level, and evaluation of the alteration should be carried out using cDNA analysis.
View Figures
View References

1 

Vogelstein B, Lane D and Levine AJ: Surfing the p53 network. Nature. 408:307–310. 2000. View Article : Google Scholar : PubMed/NCBI

2 

Hollstein M, Hergenhahn M, Yang Q, Bartsch H, Wang ZQ and Hainaut P: New approaches to understanding p53 gene tumour mutation spectra. Mutat Res. 31:199–209. 1999. View Article : Google Scholar : PubMed/NCBI

3 

Nigro JM, Baker SJ, Preisinger AC, et al: Mutations in the p53 gene occur in diverse human tumour types. Nature. 342:705–707. 1989. View Article : Google Scholar : PubMed/NCBI

4 

Laurent-Puig P, Béroud C and Soussi T: APC gene: database of germline and somatic mutations in human tumors and cell lines. Nucleic Acids Res. 26:269–270. 1998. View Article : Google Scholar : PubMed/NCBI

5 

Heim RA, Kam-Morgan LN, Binnie CG, et al: Distribution of 13 truncating mutations in the neurofibromatosis 1 gene. Hum Mol Genet. 4:975–981. 1995. View Article : Google Scholar : PubMed/NCBI

6 

Valverde JR, Alonso J, Palacios I and Pestaña A: RB1 gene mutation up-date, a meta-analysis based on 932 reported mutations available in a searchable database. BMC Genet. 6:532005. View Article : Google Scholar : PubMed/NCBI

7 

Elledge RM and Allred DC: The p53 tumour suppressor gene in breast cancer. Breast Cancer Res Treat. 32:39–47. 1994. View Article : Google Scholar : PubMed/NCBI

8 

Nakano Y, Naoe T, Kiyoi H, et al: Prognostic value of p53 gene mutations and the product expression in de novo acute myeloid leukaemia. Eur J Haematol. 65:23–31. 2000. View Article : Google Scholar : PubMed/NCBI

9 

Stirewalt DL, Kopecky KJ, Meshinchi S, Appelbaum FR, Slovak ML, Willman CL and Radich JP: FLT3, RAS and TP53 mutations in elderly patients with acute myeloid leukaemia. Blood. 97:3589–3595. 2001. View Article : Google Scholar : PubMed/NCBI

10 

Thangnipon W, Mizoguchi M, Kukita Y, Inazuka M, Iwaki T, Fukui M and Hayashi K: Distinct pattern of PCR-SSCP analysis of p53 mutations in human astrocytomas. Cancer Lett. 141:195–201. 1999. View Article : Google Scholar : PubMed/NCBI

11 

Ohgaki H, Dessen P, Jourde B, et al: Genetic pathways to glioblastoma: a population-based study. Cancer Res. 64:6892–6899. 2004. View Article : Google Scholar : PubMed/NCBI

12 

Taubert H, Würl P, Bache M, et al: The p53 gene in soft tissue sarcomas: prognostic value of DNA sequencing versus immunohistochemistry. Anticancer Res. 18:183–187. 1998.PubMed/NCBI

13 

Patterson H, Gill S, Fisher C, et al: Abnormalities of the p53 MDM2 and DCC genes in human leiomyosarcomas. Br J Cancer. 69:1052–1058. 1994. View Article : Google Scholar : PubMed/NCBI

14 

Pharaoh PD, Day NE and Caldas C: Somatic mutations in the p53 gene and prognosis in breast cancer: a meta-analysis. Br J Cancer. 80:1968–1973. 1999. View Article : Google Scholar : PubMed/NCBI

15 

Sjögren S, Inganäs M, Norberg T, Lindgren A, Nordgren H, Holmberg L and Bergh J: The p53 gene in breast cancer: prognostic value of complementary DNA sequencing versus immunohistochemistry. J Natl Cancer Inst. 88:173–182. 1996.

16 

Peraud A, Kreth FW, Wiestler OD, Kleihues P and Reulen HJ: Prognostic impact of TP53 mutations and P53 protein overexpression in supratentorial WHO grade II astrocytomas and oligoastrocytomas. Clin Cancer Res. 8:1117–1124. 2002.PubMed/NCBI

17 

Taubert H, Meye A and Würl P: Prognosis is correlated with p53 mutation type for soft tissue sarcoma patients. Cancer Res. 56:4134–4136. 1996.PubMed/NCBI

18 

Williams C, Norberg T, Ahmadian A, et al: Assessment of sequence-based p53 gene analysis in human breast cancer: messenger RNA in comparison with genomic DNA targets. Clin Chem. 44:455–462. 1998.PubMed/NCBI

19 

Forslund A, Kressner U, Lönnroth C, Andersson M, Lindmark G and Lundholm K: P53 mutations in colorectal cancer assessed in both genomic DNA and cDNA as compared to the presence of p53 LOH. Int J Oncol. 21:409–415. 2002.PubMed/NCBI

20 

Szybka M, Zawlik I, Kulczycka D, et al: Elimination of wild-type P53 mRNA in glioblastomas showing heterozygous mutations of P53. Br J Cancer. 98:1431–1433. 2008. View Article : Google Scholar : PubMed/NCBI

21 

Szybka M, Zakrzewska M, Rieske P, et al: cDNA sequencing improves the detection of P53 missense mutations in colorectal cancer. BMC Cancer. 11:2782009. View Article : Google Scholar : PubMed/NCBI

22 

Zakrzewska M, Szybka M, Biernat W, Papierz T, Rieske P, Liberski PP and Zakrzewski K: Prevalence of mutated TP53 on cDNA (but not on DNA template) in pleomorphic xanthoastrocytoma with positive TP53 immunohistochemistry. Cancer Genet Cytogenet. 193:93–97. 2009. View Article : Google Scholar : PubMed/NCBI

23 

Zakrzewska M, Wojcik I, Zakrzewski K, et al: Mutational analysis of hSNF5/INI1 and P53 genes in choro id plexus carcinomas. Cancer Genet Cytogenet. 156:179–182. 2005. View Article : Google Scholar : PubMed/NCBI

24 

Pfaffl MW, Horgan GW and Dempfle L: Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 30:e362002. View Article : Google Scholar : PubMed/NCBI

25 

Wojcik I, Szybka M, Golanska E, Rieske P, Blonski JZ, Robak T and Bartkowiak J: Abnormalities of the P53, MDM2, BCL2 and BAX genes in acute leukemias. Neoplasma. 52:318–324. 2005.PubMed/NCBI

26 

Rieske P, Bartkowiak JK, Szadowska AM, Olborski B, Harezga-Bal B and Debiec-Rychter M: A comparative study of P53/MDM2 gene alterations and P53/MDM2 protein immunoreactivity in soft-tissue sarcomas. J Exp Clin Cancer Res. 18:403–416. 1999.PubMed/NCBI

27 

Leitner T, Halapi E, Scarlatti G, Rossi P, Albert J, Fenyö EM and Uhlén M: Analysis of heterogenous viral populations by direct DNA sequencing. Bio Techniques. 15:120–126. 1993.PubMed/NCBI

28 

Singer G, Stöhr R, Cope L, et al: Patterns of p53 mutations separate ovarian serous borderline tumors and low- and high-grade carcinomas and provide support for a new model of ovarian carcinogenesis: a mutational analysis with immunohistochemical correlation. Am J Surg Pathol. 29:218–224. 2005. View Article : Google Scholar

Related Articles

  • Abstract
  • View
  • Download
  • Twitter
Copy and paste a formatted citation
Spandidos Publications style
Piaskowski S, Zawlik I, Szybka M, Kulczycka-Wojdala D, Stoczynska-Fidelus E, Bienkowski M, Robak T, Kusinska R, Jesionek-Kupnicka D, Kordek R, Kordek R, et al: Detection of P53 mutations in different cancer types is improved by cDNA sequencing . Oncol Lett 1: 717-721, 2010.
APA
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M. ... Liberski, P.P. (2010). Detection of P53 mutations in different cancer types is improved by cDNA sequencing . Oncology Letters, 1, 717-721. https://doi.org/10.3892/ol_00000125
MLA
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M., Robak, T., Kusinska, R., Jesionek-Kupnicka, D., Kordek, R., Rieske, P., Liberski, P. P."Detection of P53 mutations in different cancer types is improved by cDNA sequencing ". Oncology Letters 1.4 (2010): 717-721.
Chicago
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M., Robak, T., Kusinska, R., Jesionek-Kupnicka, D., Kordek, R., Rieske, P., Liberski, P. P."Detection of P53 mutations in different cancer types is improved by cDNA sequencing ". Oncology Letters 1, no. 4 (2010): 717-721. https://doi.org/10.3892/ol_00000125
Copy and paste a formatted citation
x
Spandidos Publications style
Piaskowski S, Zawlik I, Szybka M, Kulczycka-Wojdala D, Stoczynska-Fidelus E, Bienkowski M, Robak T, Kusinska R, Jesionek-Kupnicka D, Kordek R, Kordek R, et al: Detection of P53 mutations in different cancer types is improved by cDNA sequencing . Oncol Lett 1: 717-721, 2010.
APA
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M. ... Liberski, P.P. (2010). Detection of P53 mutations in different cancer types is improved by cDNA sequencing . Oncology Letters, 1, 717-721. https://doi.org/10.3892/ol_00000125
MLA
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M., Robak, T., Kusinska, R., Jesionek-Kupnicka, D., Kordek, R., Rieske, P., Liberski, P. P."Detection of P53 mutations in different cancer types is improved by cDNA sequencing ". Oncology Letters 1.4 (2010): 717-721.
Chicago
Piaskowski, S., Zawlik, I., Szybka, M., Kulczycka-Wojdala, D., Stoczynska-Fidelus, E., Bienkowski, M., Robak, T., Kusinska, R., Jesionek-Kupnicka, D., Kordek, R., Rieske, P., Liberski, P. P."Detection of P53 mutations in different cancer types is improved by cDNA sequencing ". Oncology Letters 1, no. 4 (2010): 717-721. https://doi.org/10.3892/ol_00000125
Follow us
  • Twitter
  • LinkedIn
  • Facebook
About
  • Spandidos Publications
  • Careers
  • Cookie Policy
  • Privacy Policy
How can we help?
  • Help
  • Live Chat
  • Contact
  • Email to our Support Team