Genetic diagnosis of patients with esophageal cancer using FISH

Awut,Idiris; Niyaz,Madiniyet; Huizhong,Xie; Biekemitoufu,Hadeti; Yan,Zhang Hong; Zhu,Zhang; Sheyhedin,Ilyar; Changmin,Zhang; Wei,Zhangli; Hao,Wen

doi:10.3892/ol_00000142

September-October 2010 Volume 1 Issue 5

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September-October 2010 Volume 1 Issue 5

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Article

Genetic diagnosis of patients with esophageal cancer using FISH

Authors:
- Idiris Awut
- Madiniyet Niyaz
- Xie Huizhong
- Hadeti Biekemitoufu
- Zhang Hong Yan
- Zhang Zhu
- Ilyar Sheyhedin
- Zhang Changmin
- Zhangli Wei
- Wen Hao
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Affiliations: Thoracic Surgery, First Affiliated Hospital of Xinjiang Medical University, Xinjiang 830054, P.R. China
Pages: 809-813
|
Published online on: September 1, 2010

https://doi.org/10.3892/ol_00000142
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Abstract

This study aimed to the clarify the diagnostic efficacy of fluorescence in situ hybridization (FISH) in Kazakh patients with esophageal cancer (EC). FISH was compared with the pathological examination of biopsy specimens with DNA probes. We enrolled 20 patients, of which 15 were males and 5 females, with an average age of 58.3 years, who had abnormal esophaguses on barium radiological digital imaging. Touch preparations were performed on biopsy specimens from all of the patients and were examined using FISH for chromosomal abnormalities. We compared the FISH results with the pathology slides stained with hematoxylin and eosin. Classification, according to pathology, identified 2 cases of class II, 3 cases of IIIa, 1 case of IIIb, 2 cases of IV, 12 cases of class V and no cases of class I. The cases classified as class IIIb or higher were considered to be positive for cancer. Using histopathology, 10 cases were diagnosed with squamous cell carcinoma and 5 were diagnosed as adenocarcinoma, with one case being false-negative. Thus, the sensitivity of the pathological examination was 93% and the specificity was 100%. Using FISH, 16 cases showed aberrant copy numbers in either chromosome 3 or 17. By comparison, pathology did not reveal any false-positive or false-negative cases with a sensitivity and specificity of 100%. The centromeres of chromosome 3 copy numbers was significantly higher (p=0.035) than the centromeres of chromosome 17. Our study compared FISH to diagnose aneusomic esophageal cancer cells with the pathology of biopsied tissue. Our findings suggest that FISH is a useful and objective assay for the detection of malignant cells of esophageal cancer. In our study, the centromeres of chromosome 3 was the more sensitive probe for the diagnosis of esophageal cancer in Kazakh patients.

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Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Genetic diagnosis of patients with esophageal cancer using FISH

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Abstract

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