Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro

  • Authors:
    • Xun Tian
    • Wei Wang
    • Qinghua Zhang
    • Liangping Zhao
    • Junchen Wei
    • Hui Xing
    • Yuping Song
    • Shixuan Wang
    • Ding Ma
    • Li Meng
    • Gang Chen
  • View Affiliations

  • Published online on: September 1, 2010     https://doi.org/10.3892/ol_00000159
  • Pages: 893-897
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Abstract

The purpose of this study was to clarify the direct effect of hypoxia-inducible factor-1α (HIF-1α) on tumor growth, apoptosis and migration in vitro. To achieve this aim, a comparison was made of the differences in growth rates, apoptotic indices and cell invasive ability in the human cervical cancer cell line HeLa and the HIF-1α-blocked counterpart in a three-dimensional spheroid culture. A significant decrease in cell proliferation and invasion, and an increase in cell apoptosis were observed in HIF-1α-blocked cells in the three-dimensional culture. The data indicated that a multicellular spheroid culture is an ideal model of hypoxia in vitro and that HIF-1α is a significant regulator of adaptive processes that promote tumor cell malignant phenotypes, such as proliferation, anti-apoptosis and invasive ability.
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September-October 2010
Volume 1 Issue 5

Print ISSN: 1792-1074
Online ISSN:1792-1082

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Spandidos Publications style
Tian X, Wang W, Zhang Q, Zhao L, Wei J, Xing H, Song Y, Wang S, Ma D, Meng L, Meng L, et al: Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro . Oncol Lett 1: 893-897, 2010
APA
Tian, X., Wang, W., Zhang, Q., Zhao, L., Wei, J., Xing, H. ... Chen, G. (2010). Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro . Oncology Letters, 1, 893-897. https://doi.org/10.3892/ol_00000159
MLA
Tian, X., Wang, W., Zhang, Q., Zhao, L., Wei, J., Xing, H., Song, Y., Wang, S., Ma, D., Meng, L., Chen, G."Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro ". Oncology Letters 1.5 (2010): 893-897.
Chicago
Tian, X., Wang, W., Zhang, Q., Zhao, L., Wei, J., Xing, H., Song, Y., Wang, S., Ma, D., Meng, L., Chen, G."Hypoxia-inducible factor-1α enhances the malignant phenotype of multicellular spheroid HeLa cells in vitro ". Oncology Letters 1, no. 5 (2010): 893-897. https://doi.org/10.3892/ol_00000159