THE MICROFOCUS ASSAY SYSTEM (MFAS) - A SIMPLE QUANTITATIVE ASSAY TO IDENTIFY INHIBITORY DRUGS TARGETED AGAINST SPECIFIC ONCOGENES

  • Authors:
    • DB REARDON
    • YM XIE
    • MC HUNG
  • View Affiliations

  • Published online on: January 1, 1994     https://doi.org/10.3892/or.1.1.37
  • Pages: 37-41
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Abstract

We have developed an assay, the microfocus assay system (MFAS), that enables the quantitative assessment of anticancer drugs and their ability to specifically target and inhibit cellular oncogenes. Analysis of three chemotherapeutic reagents showed a differential negative effect against the ability of transformed cells containing either the oncogenic rat neu, human H-ras, or v-mos oncogene to form transformed foci on a background of normal fibroblasts. Though these drugs have not been examined previously for targeting against specific oncogenes, our results with the MFAS indicate preferential inhibitory effects against cells transformed with different oncogenes. Therefore, the MFAS should enable the large scale screening of anti-cancer drugs specifically targeted against known oncogenes and provide a quantitative measurement of their effectiveness.

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January 1994
Volume 1 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
REARDON D, XIE Y and HUNG M: THE MICROFOCUS ASSAY SYSTEM (MFAS) - A SIMPLE QUANTITATIVE ASSAY TO IDENTIFY INHIBITORY DRUGS TARGETED AGAINST SPECIFIC ONCOGENES. Oncol Rep 1: 37-41, 1994.
APA
REARDON, D., XIE, Y., & HUNG, M. (1994). THE MICROFOCUS ASSAY SYSTEM (MFAS) - A SIMPLE QUANTITATIVE ASSAY TO IDENTIFY INHIBITORY DRUGS TARGETED AGAINST SPECIFIC ONCOGENES. Oncology Reports, 1, 37-41. https://doi.org/10.3892/or.1.1.37
MLA
REARDON, D., XIE, Y., HUNG, M."THE MICROFOCUS ASSAY SYSTEM (MFAS) - A SIMPLE QUANTITATIVE ASSAY TO IDENTIFY INHIBITORY DRUGS TARGETED AGAINST SPECIFIC ONCOGENES". Oncology Reports 1.1 (1994): 37-41.
Chicago
REARDON, D., XIE, Y., HUNG, M."THE MICROFOCUS ASSAY SYSTEM (MFAS) - A SIMPLE QUANTITATIVE ASSAY TO IDENTIFY INHIBITORY DRUGS TARGETED AGAINST SPECIFIC ONCOGENES". Oncology Reports 1, no. 1 (1994): 37-41. https://doi.org/10.3892/or.1.1.37