DEPHOSPHORYLATION OF RETINOBLASTOMA PROTEIN-INDUCED BY GAMMA-RADIATION CORRELATES WITH CELL-CYCLE PERTURBATION

  • Authors:
    • LP CHANG
    • FYH WU
  • View Affiliations

  • Published online on: May 1, 1994     https://doi.org/10.3892/or.1.3.503
  • Pages: 503-506
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Abstract

Gamma radiation is a well-known antiproliferative agent, yet its mechanism of inhibiting tumor cell growth is not fully understood. Human oral epidermoid carcinoma KB cells were irradiated with various doses of Co-60 gamma-rays and harvested for analysis of the phosphorylation status of retinoblastoma protein (Rb) by SDS-polyacrylamide gel electrophoresis and analysis of their DNA content by flow cytometry. Dephosphorylation of Rb, which coincided with S and G2 arrest was observed in the lethal dose range of 750-1500 cGy. Both effects could be reversed by 1 mM caffeine, an inhibitor of tyrosine kinase and DNA repair enzymes. These results suggest that gamma-radiation, like many other antiproliferative agents, induces cell cycle arrest and growth inhibition in which dephosphorylation or reactivation of Rb plays an important role.

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May 1994
Volume 1 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
CHANG L and CHANG L: DEPHOSPHORYLATION OF RETINOBLASTOMA PROTEIN-INDUCED BY GAMMA-RADIATION CORRELATES WITH CELL-CYCLE PERTURBATION. Oncol Rep 1: 503-506, 1994
APA
CHANG, L., & CHANG, L. (1994). DEPHOSPHORYLATION OF RETINOBLASTOMA PROTEIN-INDUCED BY GAMMA-RADIATION CORRELATES WITH CELL-CYCLE PERTURBATION. Oncology Reports, 1, 503-506. https://doi.org/10.3892/or.1.3.503
MLA
CHANG, L., WU, F."DEPHOSPHORYLATION OF RETINOBLASTOMA PROTEIN-INDUCED BY GAMMA-RADIATION CORRELATES WITH CELL-CYCLE PERTURBATION". Oncology Reports 1.3 (1994): 503-506.
Chicago
CHANG, L., WU, F."DEPHOSPHORYLATION OF RETINOBLASTOMA PROTEIN-INDUCED BY GAMMA-RADIATION CORRELATES WITH CELL-CYCLE PERTURBATION". Oncology Reports 1, no. 3 (1994): 503-506. https://doi.org/10.3892/or.1.3.503