ISOLATION OF TUMOR-ASSOCIATED PROTEIN FROM SERA OF COLON TUMOR-BEARING RATS USING POLYCLONAL ANTIBODIES ENTRAPPED IN GEL FIBERGLASS COLUMNS
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- Published online on: July 1, 1994 https://doi.org/10.3892/or.1.4.751
- Pages: 751-754
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Abstract
A new, effective support for the isolation of proteins has been invented utilizing a gel fiberglass (GFG) (R. Zusman, Patent application 1992, 1993). We describe the utilization of this support to isolate tumor-associated proteins (TAP) from sera of colon tumor-bearing rats. Sera were percolated through GFG columns with entrapped anti-rat colon cancer IgG from rabbits. TAP were eluted in a large amount, up to 3 mg/ml sera/column. Their affinity to antitumorous polyclonal antibodies was detected by ELISA. Western immunoblotting with commercial monoclonal antibodies has identified the isolated antigens as p21 and p53. Their concentrations were much higher (up to 1,000 times) in sera from tumor-bearing rats compared to control rats. The method was highly reproducible. It is suggested that data obtained can be considered as a basis for the further improving of quantitative methods of diagnosis.
