search button

LEVELS OF MESSENGER-RNA CODING FOR DNA TOPOISOMERASE-II ISOFORMS DO NOT CORRELATE WITH IN-VITRO DRUG-SENSITIVITY

  • Authors:
    • AC PETERS
    • AM SMYTHE
    • L WU
    • A MONKS
    • MR BOYD
    • RH SHOEMAKER

  • View Affiliations

  • Published online on: September 1, 1994     https://doi.org/10.3892/or.1.5.907
  • Pages: 907-911
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

The relationship between cellular levels of mRNA coding for DNA topoisomerase II, both the alpha and beta isoforms, and in vitro sensitivity to anticancer drugs were evaluated. Using a sensitive RNA-polymerase chain reaction technique, the levels of mRNA coding for the alpha and beta isoforms of topoisomerase II were estimated relative to beta-actin mRNA. A relatively narrow range of expression was observed across a broad range of approximately 60 human tumor cell lines representing eight major histological types which have been characterized in detail with respect to their in vitro sensitivity to standard anticancer drugs. No significant correlations were observed between mRNA level and cellular response to drugs thought to inhibit topoisomerase II or any of the other drugs studied. These results suggest that predictive tests for response to topoisomerase II-related drugs can not be based on estimation of levels of mRNA.

Related Articles

Journal Cover

September 1994
Volume 1 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

  • Journal Metrics
  • Impact Factor: 4.2
  • Ranked Oncology
    (total number of cites)
  • CiteScore: 8.4
  • CiteScore Rank: Q1: #68/366 Oncology
  • Source Normalized Impact per Paper (SNIP): 0.786
  • SCImago Journal Rank (SJR): 0.812
International Symposium on Molecular Medicine Conference Banner
The Cancer Story
World Congress on Advances in Oncology and International Symposium on Molecular Medicine Conference Banner
World Academy Of Sciences Banner
Heyday Road Banner

0

Copy and paste a formatted citation
x
Spandidos Publications style
PETERS A, SMYTHE A, WU L, MONKS A, BOYD M and SHOEMAKER R: LEVELS OF MESSENGER-RNA CODING FOR DNA TOPOISOMERASE-II ISOFORMS DO NOT CORRELATE WITH IN-VITRO DRUG-SENSITIVITY. Oncol Rep 1: 907-911, 1994
APA
PETERS, A., SMYTHE, A., WU, L., MONKS, A., BOYD, M., & SHOEMAKER, R. (1994). LEVELS OF MESSENGER-RNA CODING FOR DNA TOPOISOMERASE-II ISOFORMS DO NOT CORRELATE WITH IN-VITRO DRUG-SENSITIVITY. Oncology Reports, 1, 907-911. https://doi.org/10.3892/or.1.5.907
MLA
PETERS, A., SMYTHE, A., WU, L., MONKS, A., BOYD, M., SHOEMAKER, R."LEVELS OF MESSENGER-RNA CODING FOR DNA TOPOISOMERASE-II ISOFORMS DO NOT CORRELATE WITH IN-VITRO DRUG-SENSITIVITY". Oncology Reports 1.5 (1994): 907-911.
Chicago
PETERS, A., SMYTHE, A., WU, L., MONKS, A., BOYD, M., SHOEMAKER, R."LEVELS OF MESSENGER-RNA CODING FOR DNA TOPOISOMERASE-II ISOFORMS DO NOT CORRELATE WITH IN-VITRO DRUG-SENSITIVITY". Oncology Reports 1, no. 5 (1994): 907-911. https://doi.org/10.3892/or.1.5.907