Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120

  • Authors:
    • Wolfgang Hilbe
    • Anne Gächter
    • Hans-Christoph Duba
    • Stephan Dirnhofer
    • Wolfgang Eisterer
    • Thomas Schmid
    • Andreas Mildner
    • Johannes Bodner
    • Ewald Wöll
  • View Affiliations

  • Published online on: January 1, 2003     https://doi.org/10.3892/or.10.1.15
  • Pages: 15-20
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Abstract

Seventy-one frozen lung tissue specimens from 52 patients suffering from non-small cell lung cancer (NSCLC) were analysed in this study. Cryostat sections were stained with monoclonal antibodies against p53, ki-67 and p120, all of which are of prognostic value in NSCLC. Slides were evaluated by standard manual microscopy (MM) and using a new Automated Cellular Imaging System (ACIS). The results obtained with ACIS correlated significantly with MM examination (p<0.001). However, ACIS showed a higher sensitivity, especially for specimens with a low infiltration volume. In 15 (6.8%) MM negative cases singular positive cells were identified with ACIS. In cases with a high infiltration volume subjective (MM) quantitation tended to overestimate the number of infiltrating cells. ACIS guaranteed a high reproducibility of data. We conclude that ACIS-assisted analysis is a valid means of investigating the p53, ki-67 and p120 antibody expression in cryostat sections of lung cancer specimens. ACIS can complement conventional manual microscopy due to its higher accuracy, sensitivity and better reproducibility of data.

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January-February 2003
Volume 10 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hilbe W, Gächter A, Duba H, Dirnhofer S, Eisterer W, Schmid T, Mildner A, Bodner J and Wöll E: Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120. Oncol Rep 10: 15-20, 2003
APA
Hilbe, W., Gächter, A., Duba, H., Dirnhofer, S., Eisterer, W., Schmid, T. ... Wöll, E. (2003). Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120. Oncology Reports, 10, 15-20. https://doi.org/10.3892/or.10.1.15
MLA
Hilbe, W., Gächter, A., Duba, H., Dirnhofer, S., Eisterer, W., Schmid, T., Mildner, A., Bodner, J., Wöll, E."Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120". Oncology Reports 10.1 (2003): 15-20.
Chicago
Hilbe, W., Gächter, A., Duba, H., Dirnhofer, S., Eisterer, W., Schmid, T., Mildner, A., Bodner, J., Wöll, E."Comparison of automated cellular imaging system and manual microscopy for immunohistochemically stained cryostat sections of lung cancer specimens applying p53, ki-67 and p120". Oncology Reports 10, no. 1 (2003): 15-20. https://doi.org/10.3892/or.10.1.15