Reduction of dihydrodiol dehydrogenase expression in resected hepatocellular carcinoma

  • Authors:
    • Mei-Due Yang
    • Cheng-Chung Wu
    • Shiow-Her Chiou
    • Chang-Fang Chiu
    • Tze-Yi Lin
    • I-Ping Chiang
    • Kuan-Chih Chow
  • View Affiliations

  • Published online on: Saturday, March 1, 2003
  • Pages:271-276 DOI: 10.3892/or.10.2.271
0

Abstract

Dihydrodiol dehydrogenase (DDH) is one of the major enzymes catabolizing polycyclic aromatic hydrocarbons in the liver. Although four DDH isoforms have been detected in the normal liver, only DDH1 and DDH2 have been detected in cancer cells of lung and esophagus. Moreover, the available information about hepatic pathophysiological regulation of DDH expression is limited. Therefore we addressed the question of DDH expression in patients with liver disorders, in particular, patients with hepatocellular carcinoma (HCC). Expression of DDH1/2 was determined by immunohistochemistry, immunoblotting and reverse transcription-polymerase chain reaction (RT-PCR) in 52 patients with resected HCC. DDH1/2 expression was detected in 31 (59.6%) of 52 pathological sections. Frequency of DDH1/2 expression was significantly higher in patients with tumor size >2 cm, and in those who had early local recurrence. In addition to the tumor size and frequency of local recurrence, our results further indicated that expression of DDH1/2 was correlated with those of cyclooxygenase 2 (COX-2), interleukin-6 (IL-6), microsomal epoxide hydrolase (mEpH) and soluble epoxide hydrolase (sEpH) in HCC patients. Interestingly, the expression of DDH1/2 was found inversely correlated with that of glutathione S-transferase (GST) and NADPH p450 reductase (NPR). In conclusion, these results indicate that DDH expression was significantly decreased in about 40% of HCC patients. However, in the bordering non-neoplastic region of liver DDH1/2 expression increased, and the increased DDH1/2 expression correlated with tumor size and the disease progression.

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March 2003
Volume 10 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

2015 Impact Factor: 2.486
Ranked #31/211 Oncology
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APA
Yang, M., Wu, C., Chiou, S., Chiu, C., Lin, T., Chiang, I., & Chow, K. (2003). Reduction of dihydrodiol dehydrogenase expression in resected hepatocellular carcinoma. Oncology Reports, 10, 271-276. http://dx.doi.org/10.3892/or.10.2.271
MLA
Yang, M., Wu, C., Chiou, S., Chiu, C., Lin, T., Chiang, I., Chow, K."Reduction of dihydrodiol dehydrogenase expression in resected hepatocellular carcinoma". Oncology Reports 10.2 (2003): 271-276.
Chicago
Yang, M., Wu, C., Chiou, S., Chiu, C., Lin, T., Chiang, I., Chow, K."Reduction of dihydrodiol dehydrogenase expression in resected hepatocellular carcinoma". Oncology Reports 10, no. 2 (2003): 271-276. http://dx.doi.org/10.3892/or.10.2.271