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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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May-June 2003 Volume 10 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Article

DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples

  • Authors:
    • Adriana De Capoa
    • Angelina Musolino
    • Simonetta Della Rosa
    • Paola Caiafa
    • Luciano Mariani
    • Franca Del Nonno
    • Amina Vocaturo
    • Raffaele Perrone Donnorso
    • Alain Niveleau
    • Claudio Grappelli

  • View Affiliations / Copyright

    Affiliations: Department of Genetics and Molecular Biology, Universita di Roma 1, La Sapienza, 00185 Roma, Italy. adriana.decapoa@uniroma1.it
  • Pages: 545-549
    |
    Published online on: May 1, 2003
       https://doi.org/10.3892/or.10.3.545
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Abstract

A computer-assisted assay based on the quantitative analysis of DNA methylation in individual interphase nuclei by indirect immunolabelling with anti-5-methylcytosine antibodies was recently developed in our laboratory. In situ analyses were performed on individual nuclei from normal and experimentally hypo- or hypermethylated cultured cells as well as on human peripheral blood B-lymphocytes from normal and chronic lymphoid leukemia (CLL) samples. We present the results obtained on cells from patients affected by different degrees of preneoplastic or neoplastic changes of the uterine cervix as compared to normal controls. The analysis of DNA methylation in individual cells from cytofuge samples was performed as follows: within each nucleus the eu- and heterochromatin methylation levels were quantified in the grey scale range by dedicated software in terms of numbers, areas and optical densities (ODs) of the immunolabeled dense heterochromatic regions (‘spots’), and of the optical density of nuclear background, i.e., of nuclear euchromatin. Analogously, in randomly chosen microscope fields of tissue sections from paraffin-embedded samples, progressive tissue demethylation was observed in dysplastic and cancer cells as compared to normal ones. Both methods showed significant and progressive DNA hypomethylation in dysplastic and cancer cells as compared to control specimens.

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Copy and paste a formatted citation
Spandidos Publications style
De Capoa A, Musolino A, Della Rosa S, Caiafa P, Mariani L, Del Nonno F, Vocaturo A, Perrone Donnorso R, Niveleau A, Grappelli C, Grappelli C, et al: DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. Oncol Rep 10: 545-549, 2003.
APA
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F. ... Grappelli, C. (2003). DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. Oncology Reports, 10, 545-549. https://doi.org/10.3892/or.10.3.545
MLA
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F., Vocaturo, A., Perrone Donnorso, R., Niveleau, A., Grappelli, C."DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples". Oncology Reports 10.3 (2003): 545-549.
Chicago
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F., Vocaturo, A., Perrone Donnorso, R., Niveleau, A., Grappelli, C."DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples". Oncology Reports 10, no. 3 (2003): 545-549. https://doi.org/10.3892/or.10.3.545
Copy and paste a formatted citation
x
Spandidos Publications style
De Capoa A, Musolino A, Della Rosa S, Caiafa P, Mariani L, Del Nonno F, Vocaturo A, Perrone Donnorso R, Niveleau A, Grappelli C, Grappelli C, et al: DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. Oncol Rep 10: 545-549, 2003.
APA
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F. ... Grappelli, C. (2003). DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples. Oncology Reports, 10, 545-549. https://doi.org/10.3892/or.10.3.545
MLA
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F., Vocaturo, A., Perrone Donnorso, R., Niveleau, A., Grappelli, C."DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples". Oncology Reports 10.3 (2003): 545-549.
Chicago
De Capoa, A., Musolino, A., Della Rosa, S., Caiafa, P., Mariani, L., Del Nonno, F., Vocaturo, A., Perrone Donnorso, R., Niveleau, A., Grappelli, C."DNA demethylation is directly related to tumour progression: Evidence in normal, pre-malignant and malignant cells from uterine cervix samples". Oncology Reports 10, no. 3 (2003): 545-549. https://doi.org/10.3892/or.10.3.545
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