Effect of hepatocyte growth factor on invasion of prostate cancer cell lines

  • Authors:
    • Yasuyoshi Fujiuchi
    • Osamu Nagakawa
    • Koji Murakami
    • Hideki Fuse
    • Ikuo Saiki
  • View Affiliations

  • Published online on: July 1, 2003     https://doi.org/10.3892/or.10.4.1001
  • Pages: 1001-1006
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Abstract

Hepatocyte growth factor (HGF) was suggested to play an important role in the regulation of mitogenesis, motogenesis, angiogenesis, migration and invasion for various types of cells, and acts through a specific membrane receptor encoded by c-met proto-oncogene. However, the mechanism of the effect of HGF on tumor invasion of prostate cancer cells remains unclear. We investigated the effect of HGF on the invasion of PC-3 and DU-145 prostate cancer cells through a reconstituted basement membrane (Matrigel), the haptotactic migration to fibronectin substrate, the expression of protein and mRNA for matrix metalloproteinases (MMP)-1 and -9, membrane-type 1-MMP (MT1-MMP), urokinase-type plasminogen activator (u-PA) and its receptor (uPAR). HGF increased both Matrigel invasion and haptotactic migration of prostate cancer cells. Furthermore, HGF also increased the production of MMP-1 and -9, MT1-MMP, u-PA and uPAR of these cells. These results suggested that HGF increased the invasive potential of prostate cancer cells probably through enhancement of cell motility and the production of MMPs and u-PA.

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July 2003
Volume 10 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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APA
Fujiuchi, Y., Nagakawa, O., Murakami, K., Fuse, H., & Saiki, I. (2003). Effect of hepatocyte growth factor on invasion of prostate cancer cell lines. Oncology Reports, 10, 1001-1006. https://doi.org/10.3892/or.10.4.1001
MLA
Fujiuchi, Y., Nagakawa, O., Murakami, K., Fuse, H., Saiki, I."Effect of hepatocyte growth factor on invasion of prostate cancer cell lines". Oncology Reports 10.4 (2003): 1001-1006.
Chicago
Fujiuchi, Y., Nagakawa, O., Murakami, K., Fuse, H., Saiki, I."Effect of hepatocyte growth factor on invasion of prostate cancer cell lines". Oncology Reports 10, no. 4 (2003): 1001-1006. https://doi.org/10.3892/or.10.4.1001