Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization

  • Authors:
    • Maciej Malecki
    • Magdalena Seneta
    • Joanna Miloszewska
    • Halina Trembacz
    • Malgorzata Przybyszewska
    • Przemyslaw Janik
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/or.11.1.161
  • Pages: 161-165
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Abstract

In this work we studied the effect of v-raf and different form RAF on up regulation of VEGF gene expression and angiogenesis. In the experiments the truncated forms of RAF1 gene were transfected into the cells of the established human urothelial line HCV-29, otherwise non-angiogenic. The plasmids coding for mitochondrial targeted dominant active or dominant negative RAF1 (DAM, DNM respectively) were deleted with RAS binding domain. DAM has the ability to phosphorylate BAD whereas DNM cannot phosphorylate BAD. DAM RAF1, similarly to v-raf transfected cells, induced up-regulation of VEGF and caused angiogenic phenotype as studied in vivo. DNM RAF1 transfectants induced VEGF independent angiogenesis.

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January 2004
Volume 11 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Malecki M, Seneta M, Miloszewska J, Trembacz H, Przybyszewska M and Janik P: Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization. Oncol Rep 11: 161-165, 2004
APA
Malecki, M., Seneta, M., Miloszewska, J., Trembacz, H., Przybyszewska, M., & Janik, P. (2004). Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization. Oncology Reports, 11, 161-165. https://doi.org/10.3892/or.11.1.161
MLA
Malecki, M., Seneta, M., Miloszewska, J., Trembacz, H., Przybyszewska, M., Janik, P."Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization". Oncology Reports 11.1 (2004): 161-165.
Chicago
Malecki, M., Seneta, M., Miloszewska, J., Trembacz, H., Przybyszewska, M., Janik, P."Role of v-Raf and truncated form RAF1 in the induction of vascular endothelial growth factor and vascularization". Oncology Reports 11, no. 1 (2004): 161-165. https://doi.org/10.3892/or.11.1.161