Alterations of transforming growth factor β receptor II, insulin growth factor receptor II genes in microsatellite unstable prostate carcinomas

  • Authors:
    • Nandan Bhattacharyya
    • Jianming Tao
    • Eric A. Klein
    • Sipra Banerjee
  • View Affiliations

  • Published online on: January 1, 2004     https://doi.org/10.3892/or.11.1.231
  • Pages: 231-236
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Abstract

DNA from 45 primary prostate tumors and corresponding normal tissues were analyzed to detect whether the alterations of transforming growth factor β receptor II (TGFβRII) and insulin growth factor receptor II (IGFRII) are associated with microsatellite instability (MSI). We identified that 25 tumors were microsatellite unstable (55%). The remaining 20 tumors are found to be microsatellite stable. Loss of heterozygosity (LOH) was also tested at various loci. Results indicate that in case of TGFβRII, the rate of frame-shift mutation depends on the number of polyadenine [poly(A)] tracts. Twelve percent of the tumors had frame-shift alteration at BAT-RII locus which has 10 poly(A) repeats. Twenty percent of the tumors had frame-shift at BAT-25 locus which has 25 poly(A) repeats. In addition, IGFRII gene was examined for the presence of mutation in the repetitive sequences. Seven of the 25 tumors showed deletion of a G within eight poly(G) repeats. Besides these changes there were two tumors which showed a novel insertion of A within this poly(G) repeat making a change in 9 samples (R4, 36%). On the other hand, 4 tumors showed changes within the 5CT repeats. In addition, 3 tumors showed another novel insertion of C within the CT repeats.

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January 2004
Volume 11 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Bhattacharyya N, Tao J, Klein EA and Banerjee S: Alterations of transforming growth factor β receptor II, insulin growth factor receptor II genes in microsatellite unstable prostate carcinomas. Oncol Rep 11: 231-236, 2004
APA
Bhattacharyya, N., Tao, J., Klein, E.A., & Banerjee, S. (2004). Alterations of transforming growth factor β receptor II, insulin growth factor receptor II genes in microsatellite unstable prostate carcinomas. Oncology Reports, 11, 231-236. https://doi.org/10.3892/or.11.1.231
MLA
Bhattacharyya, N., Tao, J., Klein, E. A., Banerjee, S."Alterations of transforming growth factor β receptor II, insulin growth factor receptor II genes in microsatellite unstable prostate carcinomas". Oncology Reports 11.1 (2004): 231-236.
Chicago
Bhattacharyya, N., Tao, J., Klein, E. A., Banerjee, S."Alterations of transforming growth factor β receptor II, insulin growth factor receptor II genes in microsatellite unstable prostate carcinomas". Oncology Reports 11, no. 1 (2004): 231-236. https://doi.org/10.3892/or.11.1.231