Effect of single dose irradiation on human glioblastoma spheroids in vitro

  • Authors:
    • Fabian Fehlauer
    • Lukas J.A. Stalpers
    • John Panayiotides
    • Patricia Kaaijk
    • Dionisio González González
    • Sieger Leenstra
    • Paul Van der Valk
    • Peter Sminia
  • View Affiliations

  • Published online on: February 1, 2004     https://doi.org/10.3892/or.11.2.477
  • Pages: 477-485
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Abstract

Investigation of the predictive value of a radiosurgery-relevant treatment of glioblastoma spheroids. Organotypic multicellular spheroids were cultured and irradiated (20 Gy). Morphology, apoptosis and immunohistochemical expression of p53, p21, MIB-1, TGF-β and VEGF were examined 4 h, 24 h, 7 days, and 14 days following treatment. Cell proliferation decreased, while apoptosis was increased. No morphological damage was observed. p53 expression was significantly increased after 4 h. TGF-β and VEGF expression were only slightly altered. Particularly early changes in proliferation and apoptosis can be observed in spheroids. Individual response differences suggest spheroids of human gliomas to be useful for monitoring radiosurgery effects.

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February 2004
Volume 11 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Fehlauer F, Stalpers LJ, Panayiotides J, Kaaijk P, González González D, Leenstra S, Van der Valk P and Sminia P: Effect of single dose irradiation on human glioblastoma spheroids in vitro. Oncol Rep 11: 477-485, 2004
APA
Fehlauer, F., Stalpers, L.J., Panayiotides, J., Kaaijk, P., González González, D., Leenstra, S. ... Sminia, P. (2004). Effect of single dose irradiation on human glioblastoma spheroids in vitro. Oncology Reports, 11, 477-485. https://doi.org/10.3892/or.11.2.477
MLA
Fehlauer, F., Stalpers, L. J., Panayiotides, J., Kaaijk, P., González González, D., Leenstra, S., Van der Valk, P., Sminia, P."Effect of single dose irradiation on human glioblastoma spheroids in vitro". Oncology Reports 11.2 (2004): 477-485.
Chicago
Fehlauer, F., Stalpers, L. J., Panayiotides, J., Kaaijk, P., González González, D., Leenstra, S., Van der Valk, P., Sminia, P."Effect of single dose irradiation on human glioblastoma spheroids in vitro". Oncology Reports 11, no. 2 (2004): 477-485. https://doi.org/10.3892/or.11.2.477