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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2004 Volume 11 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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February 2004 Volume 11 Issue 2

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Article

Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies

  • Authors:
    • Fabienne Grieu
    • David Joseph
    • Paul Norman
    • Barry Iacopetta

  • View Affiliations / Copyright

    Affiliations: Department of Radiation Oncology, Sir Charles Gairdner Hospital, Nedlands, Australia
  • Pages: 501-504
    |
    Published online on: February 1, 2004
       https://doi.org/10.3892/or.11.2.501
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Abstract

Single nucleotide polymorphisms (SNPs) in cancer-related genes can act as low risk genetic factors for the development of this disease. SNPs have also been shown to influence the efficacy and toxicity of various cytotoxic agents used in the treatment of cancer. Progress in these important areas of cancer research relies upon rapid, inexpensive and accurate means of SNP genotyping. In the present study we describe a fluorescent PCR-based method that utilizes single strand conformation polymorphism (SSCP) analysis to distinguish between different alleles. A real-time DNA fragment analysis platform and ultra-thin, re-useable gels allow short run times and hence a relatively high throughput to be achieved. A standardised procedure for the identification of optimal running conditions for each SNP is presented. We used this fluorescent-SSCP method to genotype SNPs in the MTHFR, p21, cyclin D1, MMP-2, vitamin D receptor, TNF-α and IL-6 genes. Multiplex PCR of two SNPs allows up to 500 genotypes per day to be evaluated with 100% accuracy. The low start-up and running costs make this method particularly well suited for SNP genotyping studies that involve up to 1,000 DNA samples.

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Copy and paste a formatted citation
Spandidos Publications style
Grieu F, Joseph D, Norman P and Iacopetta B: Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies. Oncol Rep 11: 501-504, 2004.
APA
Grieu, F., Joseph, D., Norman, P., & Iacopetta, B. (2004). Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies. Oncology Reports, 11, 501-504. https://doi.org/10.3892/or.11.2.501
MLA
Grieu, F., Joseph, D., Norman, P., Iacopetta, B."Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies". Oncology Reports 11.2 (2004): 501-504.
Chicago
Grieu, F., Joseph, D., Norman, P., Iacopetta, B."Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies". Oncology Reports 11, no. 2 (2004): 501-504. https://doi.org/10.3892/or.11.2.501
Copy and paste a formatted citation
x
Spandidos Publications style
Grieu F, Joseph D, Norman P and Iacopetta B: Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies. Oncol Rep 11: 501-504, 2004.
APA
Grieu, F., Joseph, D., Norman, P., & Iacopetta, B. (2004). Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies. Oncology Reports, 11, 501-504. https://doi.org/10.3892/or.11.2.501
MLA
Grieu, F., Joseph, D., Norman, P., Iacopetta, B."Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies". Oncology Reports 11.2 (2004): 501-504.
Chicago
Grieu, F., Joseph, D., Norman, P., Iacopetta, B."Development of a rapid genotyping method for single nucleotide polymorphisms and its application in cancer studies". Oncology Reports 11, no. 2 (2004): 501-504. https://doi.org/10.3892/or.11.2.501
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