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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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March 2004 Volume 11 Issue 3

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

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Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

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International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

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International Journal of Epigenetics

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Article

In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes

  • Authors:
    • Lori Holle
    • Wendy Song
    • Labri Hicks
    • Eric Holle
    • Lillian Holmes
    • Yanzhang Wei
    • Jinhua Li
    • Thomas Wagner
    • Xianzhong Yu

  • View Affiliations / Copyright

    Affiliations: Oncology Research Institute, Greenville Hospital System, Greenville, SC 29605, USA
  • Pages: 613-616
    |
    Published online on: March 1, 2004
       https://doi.org/10.3892/or.11.3.613
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Abstract

The NGR/α1,3Gal-HSA peptide was designed to specifically target CD13 positive cells and induce cell lysis. NGR is the targeting component of the peptide in that it binds the CD13 isoform (aminopeptidase) that is expressed in tumor vessels. Galactose α1,3-galactose terminal carbohydrate epitope (α1,3Gal) induces a strong antibody reaction in human and Old World Monkeys and in vivo, this reaction leads to organ rejection. The human serum albumin (HSA) bearing α1,3Gal epitope was therefore used to lyse cells. In the present study, we were able to demonstrate that NGR/α1,3Gal-HSA binds CD13 positive human umbilical vein endothelial cells (HUVEC). We also found by live/dead fluorescent staining that NGR/α1,3Gal-HSA was able to induce lysis of HUVECs upon incubation with human serum. Therefore, by conjugating NGR to HSA bearing α1,3Gal epitopes, we are able to specifically target and lyse cells expressing CD13. This strategy may be potentially useful in tumor anti-angiogenesis therapy.

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Copy and paste a formatted citation
Spandidos Publications style
Holle L, Song W, Hicks L, Holle E, Holmes L, Wei Y, Li J, Wagner T and Yu X: In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncol Rep 11: 613-616, 2004.
APA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y. ... Yu, X. (2004). In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncology Reports, 11, 613-616. https://doi.org/10.3892/or.11.3.613
MLA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11.3 (2004): 613-616.
Chicago
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11, no. 3 (2004): 613-616. https://doi.org/10.3892/or.11.3.613
Copy and paste a formatted citation
x
Spandidos Publications style
Holle L, Song W, Hicks L, Holle E, Holmes L, Wei Y, Li J, Wagner T and Yu X: In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncol Rep 11: 613-616, 2004.
APA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y. ... Yu, X. (2004). In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncology Reports, 11, 613-616. https://doi.org/10.3892/or.11.3.613
MLA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11.3 (2004): 613-616.
Chicago
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11, no. 3 (2004): 613-616. https://doi.org/10.3892/or.11.3.613
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