In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes

  • Authors:
    • Lori Holle
    • Wendy Song
    • Labri Hicks
    • Eric Holle
    • Lillian Holmes
    • Yanzhang Wei
    • Jinhua Li
    • Thomas Wagner
    • Xianzhong Yu
  • View Affiliations

  • Published online on: March 1, 2004     https://doi.org/10.3892/or.11.3.613
  • Pages: 613-616
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Abstract

The NGR/α1,3Gal-HSA peptide was designed to specifically target CD13 positive cells and induce cell lysis. NGR is the targeting component of the peptide in that it binds the CD13 isoform (aminopeptidase) that is expressed in tumor vessels. Galactose α1,3-galactose terminal carbohydrate epitope (α1,3Gal) induces a strong antibody reaction in human and Old World Monkeys and in vivo, this reaction leads to organ rejection. The human serum albumin (HSA) bearing α1,3Gal epitope was therefore used to lyse cells. In the present study, we were able to demonstrate that NGR/α1,3Gal-HSA binds CD13 positive human umbilical vein endothelial cells (HUVEC). We also found by live/dead fluorescent staining that NGR/α1,3Gal-HSA was able to induce lysis of HUVECs upon incubation with human serum. Therefore, by conjugating NGR to HSA bearing α1,3Gal epitopes, we are able to specifically target and lyse cells expressing CD13. This strategy may be potentially useful in tumor anti-angiogenesis therapy.

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March 2004
Volume 11 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Holle L, Song W, Hicks L, Holle E, Holmes L, Wei Y, Li J, Wagner T and Yu X: In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncol Rep 11: 613-616, 2004
APA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y. ... Yu, X. (2004). In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes. Oncology Reports, 11, 613-616. https://doi.org/10.3892/or.11.3.613
MLA
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11.3 (2004): 613-616.
Chicago
Holle, L., Song, W., Hicks, L., Holle, E., Holmes, L., Wei, Y., Li, J., Wagner, T., Yu, X."In vitro targeted killing of human endothelial cells by co-incubation of human serum and NGR peptide conjugated human albumin protein bearing α (1-3) galactose epitopes". Oncology Reports 11, no. 3 (2004): 613-616. https://doi.org/10.3892/or.11.3.613