Antineoplastic action of 5-aza-2'-deoxycytidine (Dacogen) and depsipeptide on Raji lymphoma cells

  • Authors:
    • Sepideh Shaker
    • Mark Bernstein
    • Richard L. Momparler
  • View Affiliations

  • Published online on: June 1, 2004     https://doi.org/10.3892/or.11.6.1253
  • Pages: 1253-1256
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Abstract

Epigenetic alterations, such as aberrant DNA methylation and histone deacetylation, can silence genes that suppress leukemogenesis. The objective of our study was to investigate the in vitro antineoplastic and gene re-activation activity of 5-aza-2'-deoxycytidine (5AZA), a potent inhibitor of DNA methylation, and depsipeptide (depsi), an inhibitor of histone deacetylase, on Raji lymphoma cells. The combination of 5AZA with depsi produced a significantly greater inhibition of growth and colony formation than either agent alone. Using RT-PCR, we observed that combination also produced a synergistic activation of E-cadherin, a gene that is silenced by aberrant DNA methylation in Raji cells. This latter interaction indicates that there is cross-talk between DNA methylation and histone modifications in chromatin for E-cadherin in this cell line. 5AZA and depsi may be an interesting drug combination to investigate in patients with lymphoma.

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June 2004
Volume 11 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shaker S, Bernstein M and Momparler RL: Antineoplastic action of 5-aza-2'-deoxycytidine (Dacogen) and depsipeptide on Raji lymphoma cells. Oncol Rep 11: 1253-1256, 2004
APA
Shaker, S., Bernstein, M., & Momparler, R.L. (2004). Antineoplastic action of 5-aza-2'-deoxycytidine (Dacogen) and depsipeptide on Raji lymphoma cells. Oncology Reports, 11, 1253-1256. https://doi.org/10.3892/or.11.6.1253
MLA
Shaker, S., Bernstein, M., Momparler, R. L."Antineoplastic action of 5-aza-2'-deoxycytidine (Dacogen) and depsipeptide on Raji lymphoma cells". Oncology Reports 11.6 (2004): 1253-1256.
Chicago
Shaker, S., Bernstein, M., Momparler, R. L."Antineoplastic action of 5-aza-2'-deoxycytidine (Dacogen) and depsipeptide on Raji lymphoma cells". Oncology Reports 11, no. 6 (2004): 1253-1256. https://doi.org/10.3892/or.11.6.1253