Efficiency of lipofection combined with hyperthermia in Lewis lung carcinoma cells and a rodent pleural dissemination model of lung carcinoma

Okita,Atsushi; Mushiake,Hiroyuki; Tsukuda,Kazunori; Aoe,Motoi; Murakami,Masakazu; Andou,Akio; Shimizu,Nobuyoshi

doi:10.3892/or.11.6.1313

June 2004 Volume 11 Issue 6

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June 2004 Volume 11 Issue 6

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Article

Efficiency of lipofection combined with hyperthermia in Lewis lung carcinoma cells and a rodent pleural dissemination model of lung carcinoma

Authors:
- Atsushi Okita
- Hiroyuki Mushiake
- Kazunori Tsukuda
- Motoi Aoe
- Masakazu Murakami
- Akio Andou
- Nobuyoshi Shimizu
View Affiliations / Copyright

Affiliations: Department of Cancer and Thoracic Surgery, Okayama University, Graduate School of Medicine and Dentistry, Okayama 700-8558, Japan
Pages: 1313-1318
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Published online on: June 1, 2004

https://doi.org/10.3892/or.11.6.1313
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Abstract

We have previously reported that hyperthermia at 41°C enhanced lipofection-mediated gene transduction into cultured cells. In this study, we adapted hyperthermia technique to novel cationic liposome (Lipofectamine 2000) mediated gene transfection into Lewis lung carcinoma cells in vitro and in vivo. In vitro, transfection efficiencies were 38.9±3.3% by lipofection alone and 52.1±2.6% by lipofection with hyperthermia for 30 min, and 62.5±5.5% and 81.4±3.2% for 1 h, respectively. Hyperthermia significantly enhanced gene transfection efficiency 1.2-1.4 times more than that with lipofection only. We also evaluated the effect of hyperthermia with a pleural dissemination model of lung carcinoma of mice. We developed a model which was well-tolerated with hyperthermia with lipofection by the mice. In spite of repeated treatments, transfection efficiencies were very low and we could not show the augmentation of gene transfection by hyperthermia. Though Lipofectamine 2000 showed strong gene transduction effect and hyperthermia augmented its effect in vitro, further evaluation is needed to adapt both techniques in vivo.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Efficiency of lipofection combined with hyperthermia in Lewis lung carcinoma cells and a rodent pleural dissemination model of lung carcinoma

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Abstract

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