Aberrant methylation in promoter-associated CpG islands of multiple genes in relapsed childhood acute lymphoblastic leukemia

  • Authors:
    • Chise Matsushita
    • Yang Yang
    • Seisho Takeuchi
    • Masahide Matsushita
    • Jacques J.M. Van Dongen
    • Tomasz Szczepanski
    • Claus R. Bartram
    • Hiromi Seo
    • H. Phillip Koeffler
    • Hirokuni Taguchi
  • View Affiliations

  • Published online on: July 1, 2004     https://doi.org/10.3892/or.12.1.97
  • Pages: 97-99
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Abstract

Methylation profile was analyzed in nine cases of relapsed childhood acute lymphoblastic leukemia (ALL) for p14, p15, p16, Rb, MGMT, APC, hMLH1, RARβ, RIZ, DAPK, and FHIT genes by using methylation specific polymerase chain reaction (MSP) analysis. Frequency of methylation in each gene was: MGMT, 56%; RARβ, 44%; and p16, 22%, respectively. None of the p14, p15, Rb, APC, hMLH1, RIZ, DAPK, and FHIT genes were hypermethylated. Five (56%) of 9 cases showed methylation of at least one gene. All of the samples with hypermethylation in p16 and MGMT gene at relapse, had already acquired the change at the time of initial diagnosis. Interestingly, three of 4 cases with RARβ gene methylation at relapse did not have methylation of this gene at the time of initial presentation. These results suggest that hypermethylation might be involved in the relapse of childhood ALL.

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July 2004
Volume 12 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Matsushita C, Yang Y, Takeuchi S, Matsushita M, Van Dongen JJ, Szczepanski T, Bartram CR, Seo H, Koeffler HP, Taguchi H, Taguchi H, et al: Aberrant methylation in promoter-associated CpG islands of multiple genes in relapsed childhood acute lymphoblastic leukemia. Oncol Rep 12: 97-99, 2004
APA
Matsushita, C., Yang, Y., Takeuchi, S., Matsushita, M., Van Dongen, J.J., Szczepanski, T. ... Taguchi, H. (2004). Aberrant methylation in promoter-associated CpG islands of multiple genes in relapsed childhood acute lymphoblastic leukemia. Oncology Reports, 12, 97-99. https://doi.org/10.3892/or.12.1.97
MLA
Matsushita, C., Yang, Y., Takeuchi, S., Matsushita, M., Van Dongen, J. J., Szczepanski, T., Bartram, C. R., Seo, H., Koeffler, H. P., Taguchi, H."Aberrant methylation in promoter-associated CpG islands of multiple genes in relapsed childhood acute lymphoblastic leukemia". Oncology Reports 12.1 (2004): 97-99.
Chicago
Matsushita, C., Yang, Y., Takeuchi, S., Matsushita, M., Van Dongen, J. J., Szczepanski, T., Bartram, C. R., Seo, H., Koeffler, H. P., Taguchi, H."Aberrant methylation in promoter-associated CpG islands of multiple genes in relapsed childhood acute lymphoblastic leukemia". Oncology Reports 12, no. 1 (2004): 97-99. https://doi.org/10.3892/or.12.1.97