Mobilization of peripheral blood stem cells (PBSCs) after etoposide, adriamycin and cisplatin therapy, and a multimodal cell therapy approach with PBSCs in advanced gastric cancer

  • Authors:
    • Yuji Ueda
    • Yoshiaki Sonoda
    • Hiroshi Fujiki
    • Sachio Harada
    • Takafumi Kimura
    • Tsuyoshi Itoh
    • Kenichiro Imura
    • Kei Naito
    • Kenichi Nomura
    • Masafumi Taniwaki
    • Hisakazu Yamagishi
  • View Affiliations

  • Published online on: August 1, 2004     https://doi.org/10.3892/or.12.2.323
  • Pages: 323-332
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Abstract

The EAP combination of etoposide (ETP), doxorubicin (ADM) and cisplatin (CDDP) has been reported to be highly active for advanced gastric cancer. However, it is associated with severe myelotoxicity, and its use has declined. We examined whether peripheral blood stem cells (PBSCs) could be mobilized during hematopoietic recovery after EAP, and assessed the possibility of using multimodal cell therapy with PBSCs for the treatment of advanced gastric cancer. Five men with advanced gastric adenocarcinoma were enrolled. All patients were chemotherapy-naïve. EAP (ETP, 360 mg/m2; ADM, 40 mg/m2; CDDP, 80 mg/m2) was given to each patient, and myelotoxicity was carefully monitored. Granulocyte colony-stimulating factor was administered after the neutrophil nadir, and PBSCs were collected by leuk-pheresis during hematopoietic recovery. The median nadir of the neutrophil count after EAP was 225/ml, occurring between day 17 and 20. Sufficient numbers of PBSCs [CD34(+) cells, CFU-GM] could be mobilized in 4/5 patients. A 45-year-old patient with extended lymph node metastasis received high-dose EAP with peripheral blood stem cell transplantation (PBSCT), followed by cancer vaccine therapy with dendritic cells (DCs), induced from cryopreserved PBSCs.Both high-dose EAP with PBSCT and DC-based immunotherapy was safely performed for the first time against gastric cancer. Although associated with severe myelotoxicity, EAP can mobilize sufficient numbers of PBSCs during hematopoietic recovery. Multimodal cell therapy combining high-dose chemotherapy with PBSCT and DC-based immunotherapy is feasible and can be a reasonable approach in advanced gastric cancer.

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August 2004
Volume 12 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ueda Y, Sonoda Y, Fujiki H, Harada S, Kimura T, Itoh T, Imura K, Naito K, Nomura K, Taniwaki M, Taniwaki M, et al: Mobilization of peripheral blood stem cells (PBSCs) after etoposide, adriamycin and cisplatin therapy, and a multimodal cell therapy approach with PBSCs in advanced gastric cancer. Oncol Rep 12: 323-332, 2004
APA
Ueda, Y., Sonoda, Y., Fujiki, H., Harada, S., Kimura, T., Itoh, T. ... Yamagishi, H. (2004). Mobilization of peripheral blood stem cells (PBSCs) after etoposide, adriamycin and cisplatin therapy, and a multimodal cell therapy approach with PBSCs in advanced gastric cancer. Oncology Reports, 12, 323-332. https://doi.org/10.3892/or.12.2.323
MLA
Ueda, Y., Sonoda, Y., Fujiki, H., Harada, S., Kimura, T., Itoh, T., Imura, K., Naito, K., Nomura, K., Taniwaki, M., Yamagishi, H."Mobilization of peripheral blood stem cells (PBSCs) after etoposide, adriamycin and cisplatin therapy, and a multimodal cell therapy approach with PBSCs in advanced gastric cancer". Oncology Reports 12.2 (2004): 323-332.
Chicago
Ueda, Y., Sonoda, Y., Fujiki, H., Harada, S., Kimura, T., Itoh, T., Imura, K., Naito, K., Nomura, K., Taniwaki, M., Yamagishi, H."Mobilization of peripheral blood stem cells (PBSCs) after etoposide, adriamycin and cisplatin therapy, and a multimodal cell therapy approach with PBSCs in advanced gastric cancer". Oncology Reports 12, no. 2 (2004): 323-332. https://doi.org/10.3892/or.12.2.323