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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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December 2004 Volume 12 Issue 6

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International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

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Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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December 2004 Volume 12 Issue 6

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Article

Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms

  • Authors:
    • Jiangyong Miao
    • Takeshi Kusafuka
    • Masahiro Fukuzawa

  • View Affiliations / Copyright

    Affiliations: The Department of Pediatric Surgery, Osaka University Graduate School of Medicine, Suita, Osaka 565-0871, Japan
  • Pages: 1269-1272
    |
    Published online on: December 1, 2004
       https://doi.org/10.3892/or.12.6.1269
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Abstract

BRAF (v-raf murine sarcoma viral oncogene homolog B1) activating mutations in a high proportion of melanomas and in a small fraction of other cancers have been recently reported. All the presented mutations of BRAF are located in exons 11 and 15, and the hotspot mutation at codon 599 accounts for 87% of BRAF mutations. Because the mutational status is unclear in pediatric solid neoplasms, we screened BRAF mutations comprehensively in our tumor series presented in childhood. Two pairs of primers were designed to amplify exons 11 and exon 15, respectively, and 181 tumor samples (65 neuroblastomas, 23 Wilms tumors, 19 hepatoblastomas, 16 teratomas, 17 rhabdomyosarcomas, 13 ganglioneuromas, etc.) were investigated by PCR-SSCP method. On agarose gel electrophoresis, amplified PCR fragments showed no size-altered changes in exons 11 and 15, and SSCP analysis revealed uniform band patterns in both exons. Subsequent direct sequencing of selected 10 samples confirmed only normal sequences without any nucleotide substitutions. The current study represents the first genetic analysis of the BRAF gene in pediatric solid tumors. Our data suggest that mutations of BRAF gene as a mechanism of tumorigenesis is unlikely to be associated with most childhood neoplasms.

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Copy and paste a formatted citation
Spandidos Publications style
Miao J, Kusafuka T and Fukuzawa M: Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms. Oncol Rep 12: 1269-1272, 2004.
APA
Miao, J., Kusafuka, T., & Fukuzawa, M. (2004). Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms. Oncology Reports, 12, 1269-1272. https://doi.org/10.3892/or.12.6.1269
MLA
Miao, J., Kusafuka, T., Fukuzawa, M."Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms". Oncology Reports 12.6 (2004): 1269-1272.
Chicago
Miao, J., Kusafuka, T., Fukuzawa, M."Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms". Oncology Reports 12, no. 6 (2004): 1269-1272. https://doi.org/10.3892/or.12.6.1269
Copy and paste a formatted citation
x
Spandidos Publications style
Miao J, Kusafuka T and Fukuzawa M: Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms. Oncol Rep 12: 1269-1272, 2004.
APA
Miao, J., Kusafuka, T., & Fukuzawa, M. (2004). Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms. Oncology Reports, 12, 1269-1272. https://doi.org/10.3892/or.12.6.1269
MLA
Miao, J., Kusafuka, T., Fukuzawa, M."Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms". Oncology Reports 12.6 (2004): 1269-1272.
Chicago
Miao, J., Kusafuka, T., Fukuzawa, M."Hotspot mutations of BRAF gene are not associated with pediatric solid neoplasms". Oncology Reports 12, no. 6 (2004): 1269-1272. https://doi.org/10.3892/or.12.6.1269
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