Gene expression in response to anti-tumour intervention by polysaccharide-K (PSK) in colorectal carcinoma cells

Yoshikawa,Reigetsu; Yanagi,Hidenori; Hashimoto-Tamaoki,Tomoko; Morinaga,Tomonori; Nakano,Yoshiro; Noda,Masafumi; Fujiwara,Yoshinori; Okamura,Haruki; Yamamura,Takehira

doi:10.3892/or.12.6.1287

December 2004 Volume 12 Issue 6

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December 2004 Volume 12 Issue 6

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Article

Gene expression in response to anti-tumour intervention by polysaccharide-K (PSK) in colorectal carcinoma cells

Authors:
- Reigetsu Yoshikawa
- Hidenori Yanagi
- Tomoko Hashimoto-Tamaoki
- Tomonori Morinaga
- Yoshiro Nakano
- Masafumi Noda
- Yoshinori Fujiwara
- Haruki Okamura
- Takehira Yamamura
View Affiliations / Copyright

Affiliations: Second Department of Surgery, Hyogo College of Medicine, Nishinomiya, Hyogo 663-8501, Japan. yosikr2s@hyo-med.ac.jp
Pages: 1287-1293
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Published online on: December 1, 2004

https://doi.org/10.3892/or.12.6.1287
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Abstract

Distant metastasis is one of the major problems in treatment for advanced colorectal cancer. Polysaccharide-K (PSK), or Krestin, a mushroom ingredient, has been used as a chemoimmunotherapeutic agent for the treatment of cancers in Asia for over 30 years. Some studies have reported that PSK prevent distant metastases and improve survival rates by 10-20% in colorectal cancer. However, the mechanism of the interrelated immunomodulatory and direct anti-cancer cell activities of PSK has yet to be elucidated. To investigate the direct effect, we used cDNA microarrays to analyse expression profiles in a human colorectal adenocarcinoma cell line, HCT116, containing the wild-type p53 gene. Expression of 453 genes was significantly altered (142 up-regulated and 311 down-regulated) after 96 h exposure to 500 µg/ml PSK. Under more stringent conditions, 9 genes were up-regulated and 36 down-regulated. We then examined the expression of candidate genes in two cell lines, HCT116, and SW480, a cell line with a mutant p53 gene. Our results suggest that PSK may augment anti-tumour action via genes including multidrug resistance protein 3 (MRP3), lymphotactin (Lptn), transgelin (TAGLN), and Pirin, without disturbing cell-cycle progression, and may deserve a large clinical trial in cancer therapy.

Journals

International Journal of Molecular Medicine

International Journal of Oncology

Molecular Medicine Reports

Oncology Reports

Experimental and Therapeutic Medicine

Oncology Letters

Biomedical Reports

Molecular and Clinical Oncology

World Academy of Sciences Journal

International Journal of Functional Nutrition

International Journal of Epigenetics

Medicine International

Gene expression in response to anti-tumour intervention by polysaccharide-K (PSK) in colorectal carcinoma cells

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Abstract

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