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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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February 2005 Volume 13 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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Article

Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer

  • Authors:
    • Charles Anacleto
    • Benedito Rossi
    • Ademar Lopes
    • Fernando A. Soares
    • José Cláudio C. Rocha
    • Otávia Caballero
    • Anamaria A. Camargo
    • Andrew J.G. Simpson
    • Sérgio D.J. Pena

  • View Affiliations / Copyright

    Affiliations: Departamento de Bioquímica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
  • Pages: 325-328
    |
    Published online on: February 1, 2005
       https://doi.org/10.3892/or.13.2.325
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Abstract

Cancers often exhibit aberrant methylation of gene promoter regions associated with loss of tumor suppressor and/or DNA repair gene function. Such methylation constitutes an excellent marker for the molecular detection of micro-metastases and the diagnosis of tumor recurrences. We have developed a multiplex methylation-specific PCR (MSP) procedure for rapid and simultaneous assessment of the methylation of 5 loci: the tumor suppressor genes p16INK4a, death-associated protein kinase (DAPK) and p14ARF, and the DNA repair genes hMLH1 and O6-methylguanine-DNA-methyltransferase (MGMT). This multiplex test uses one single PCR reaction and only one electrophoretic run. In 98 samples of colorectal cancer studied, methylation of MGMT, DAPK, p16, hMLH1 and p14 was present in 31, 20, 17, 16 and 14% of tumors, respectively. In 58% of the tumors at least one methylated gene was found. This multiplex MSP constitutes a simple and inexpensive method for screening of molecular signatures in colorectal cancer and can be used profitably before employing more expensive and complex techniques such as microarray testing.

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Copy and paste a formatted citation
Spandidos Publications style
Anacleto C, Rossi B, Lopes A, Soares FA, Rocha JC, Caballero O, Camargo AA, Simpson AJ and Pena SD: Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer. Oncol Rep 13: 325-328, 2005.
APA
Anacleto, C., Rossi, B., Lopes, A., Soares, F.A., Rocha, J.C., Caballero, O. ... Pena, S.D. (2005). Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer. Oncology Reports, 13, 325-328. https://doi.org/10.3892/or.13.2.325
MLA
Anacleto, C., Rossi, B., Lopes, A., Soares, F. A., Rocha, J. C., Caballero, O., Camargo, A. A., Simpson, A. J., Pena, S. D."Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer". Oncology Reports 13.2 (2005): 325-328.
Chicago
Anacleto, C., Rossi, B., Lopes, A., Soares, F. A., Rocha, J. C., Caballero, O., Camargo, A. A., Simpson, A. J., Pena, S. D."Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer". Oncology Reports 13, no. 2 (2005): 325-328. https://doi.org/10.3892/or.13.2.325
Copy and paste a formatted citation
x
Spandidos Publications style
Anacleto C, Rossi B, Lopes A, Soares FA, Rocha JC, Caballero O, Camargo AA, Simpson AJ and Pena SD: Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer. Oncol Rep 13: 325-328, 2005.
APA
Anacleto, C., Rossi, B., Lopes, A., Soares, F.A., Rocha, J.C., Caballero, O. ... Pena, S.D. (2005). Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer. Oncology Reports, 13, 325-328. https://doi.org/10.3892/or.13.2.325
MLA
Anacleto, C., Rossi, B., Lopes, A., Soares, F. A., Rocha, J. C., Caballero, O., Camargo, A. A., Simpson, A. J., Pena, S. D."Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer". Oncology Reports 13.2 (2005): 325-328.
Chicago
Anacleto, C., Rossi, B., Lopes, A., Soares, F. A., Rocha, J. C., Caballero, O., Camargo, A. A., Simpson, A. J., Pena, S. D."Development and application of a multiplex PCR procedure for the detection of DNA methylation in colorectal cancer". Oncology Reports 13, no. 2 (2005): 325-328. https://doi.org/10.3892/or.13.2.325
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