Relationship between peroxisome proliferator-activated receptor-γ expression and differentiation of human esophageal squamous cell carcinoma

  • Authors:
    • Takashi Takashima
    • Yasuhiro Fujiwara
    • Masaki Hamaguchi
    • Eiji Sasaki
    • Kazunari Tominaga
    • Toshio Watanabe
    • Nobuhide Oshitani
    • Kazuhide Higuchi
    • Tetsuo Arakawa
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/or.13.4.601
  • Pages: 601-606
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Abstract

Peroxisome proliferator-activated receptor-gamma (PPAR-γ), a member of the nuclear hormone receptor superfamily, is involved in suppressing the growth of several tumors. We showed that PPAR-γ is expressed in Barrett's adenocarcinoma cell lines and inhibited the growth of these lines through the induction of G1 cell cycle arrest and apoptosis. We examined PPAR-γ expression in human esophageal squamous cell carcinoma (SCC) in vitro and in vivo and investigated whether PPAR-γ ligands affect the proliferation and apoptosis of human SCC cell lines. Biopsy specimens (n=46) obtained from human SCC of the esophagus were stained using a monoclonal antibody against human PPAR-γ. We assessed the effects of PPAR-γ ligands on the growth of SCC cells by adding 15-deoxy prostaglandin J2 (15d-PGJ2), or troglitazone to six human esophageal SCC cell lines (TE-1, TE-2, TE-3, TE-5, TE-8, and TE-9). Immunohistochemical staining showed that 34 of 46 (73.9%) SCC of the esophagus expressed PPAR-γ. All SCC cell lines expressed PPAR-γ mRNA and protein, especially when poorly differentiated (TE-2, TE-5, and TE-9). The PPAR-γ ligands significantly and dose-dependently inhibited the proliferation of SCC lines, except for well-differentiated TE-1 and TE-3. Apoptosis was induced by 15d-PGJ2 (10 µM) in all tested SCC lines except TE-1, whereas troglitazone (50 µM) was marginally effective in only the TE-2 and TE-3 cell lines. The present findings suggest that PPAR-γ could be a therapeutic target for treating squamous cell carcinoma of the esophagus, possibly through the induction of apoptosis.

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April 2005
Volume 13 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Takashima T, Fujiwara Y, Hamaguchi M, Sasaki E, Tominaga K, Watanabe T, Oshitani N, Higuchi K and Arakawa T: Relationship between peroxisome proliferator-activated receptor-γ expression and differentiation of human esophageal squamous cell carcinoma. Oncol Rep 13: 601-606, 2005.
APA
Takashima, T., Fujiwara, Y., Hamaguchi, M., Sasaki, E., Tominaga, K., Watanabe, T. ... Arakawa, T. (2005). Relationship between peroxisome proliferator-activated receptor-γ expression and differentiation of human esophageal squamous cell carcinoma. Oncology Reports, 13, 601-606. https://doi.org/10.3892/or.13.4.601
MLA
Takashima, T., Fujiwara, Y., Hamaguchi, M., Sasaki, E., Tominaga, K., Watanabe, T., Oshitani, N., Higuchi, K., Arakawa, T."Relationship between peroxisome proliferator-activated receptor-γ expression and differentiation of human esophageal squamous cell carcinoma". Oncology Reports 13.4 (2005): 601-606.
Chicago
Takashima, T., Fujiwara, Y., Hamaguchi, M., Sasaki, E., Tominaga, K., Watanabe, T., Oshitani, N., Higuchi, K., Arakawa, T."Relationship between peroxisome proliferator-activated receptor-γ expression and differentiation of human esophageal squamous cell carcinoma". Oncology Reports 13, no. 4 (2005): 601-606. https://doi.org/10.3892/or.13.4.601