Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma

  • Authors:
    • Ken Nakatani
    • Megumi Nakamura
    • Katsuhiro Uzawa
    • Takeshi Wada
    • Naohiko Seki
    • Hideki Tanzawa
    • Shigeyuki Fujita
  • View Affiliations

  • Published online on: April 1, 2005     https://doi.org/10.3892/or.13.4.709
  • Pages: 709-714
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Abstract

Cisplatin (CDDP) is widely used for chemotherapy of many malignancies, especially of oral squamous cell carcinoma (SCC). However, because the mechanism of resistance to CDDP is unclear, we established a CDDP-resistant cell line, Sa-3R, from a CDDP-sensitive cell line, Sa-3, which was derived from moderately differentiated SCC of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide assay indicated that Sa-3R has 7.5-fold greater resistance to CDDP than Sa-3. Comparing gene expression levels in the cell lines using an in-house cDNA microarray, which represented 2,201 oral disease origin genes, many differentially expressed genes were identified. The ATP-binding cassette transporter genes (MDR-1, MRP-1, and MRP-2), and FANCONI, GRP58, FLJ12089, and SPINT-2 were up-regulated, whereas FOSL1, MRPS27, and PGK-1 were down-regulated. These results were confirmed by semiquantitative reverse transcriptase-polymerase chain reaction. The Sa-3/Sa-3R cell lines could be useful to identify the candidates responsible for the mechanism of CDDP-resistance and the up- or down-regulated genes identified by the gene expression profiles in the Sa-3R cell line may be, in part, associated with the mechanism.

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April 2005
Volume 13 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nakatani K, Nakamura M, Uzawa K, Wada T, Seki N, Tanzawa H and Fujita S: Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma. Oncol Rep 13: 709-714, 2005
APA
Nakatani, K., Nakamura, M., Uzawa, K., Wada, T., Seki, N., Tanzawa, H., & Fujita, S. (2005). Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma. Oncology Reports, 13, 709-714. https://doi.org/10.3892/or.13.4.709
MLA
Nakatani, K., Nakamura, M., Uzawa, K., Wada, T., Seki, N., Tanzawa, H., Fujita, S."Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma". Oncology Reports 13.4 (2005): 709-714.
Chicago
Nakatani, K., Nakamura, M., Uzawa, K., Wada, T., Seki, N., Tanzawa, H., Fujita, S."Establishment and gene analysis of a cisplatin-resistant cell line, Sa-3R, derived from oral squamous cell carcinoma". Oncology Reports 13, no. 4 (2005): 709-714. https://doi.org/10.3892/or.13.4.709