Differentiation and apoptosis in human malignant melanoma G-361 cells induced by 2-aminophenoxazine-3-one

  • Authors:
    • Shigetaka Shimizu
    • Mamoru Suzuki
    • Keisuke Miyazawa
    • Tomohisa Yokoyama
    • Kazuma Ohyashiki
    • Kaori Miyazaki
    • Akihisa Abe
    • Akio Tomoda
  • View Affiliations

  • Published online on: July 1, 2005     https://doi.org/10.3892/or.14.1.41
  • Pages: 41-46
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Abstract

Inhibitory effects of 2-amino-4,4α-dihydro-4α,7-dimethyl-3H-phenoxazine-3-one (Phx-1), 2-amino-4,4α-dihydro-4α,7-dimethyl-3H-phenoxazine-3-one (Phx-2) and 2-aminophenoxazine-3-one (Phx-3), which were produced by the reaction of o-aminophenol and its derivatives with bovine hemoglobin, on the proliferation of human malignant melanoma G-361 cells were studied under various conditions. Phx-1 and Phx-3 showed anti-proliferative effects on human malignant melanoma G-361 cells, however Phx-2 did not. Phx-3, which exerted the strongest anti-proliferative effects, inhibited the proliferation of human malignant melanoma G-361 cells during 24 h incubation at concentrations of ≥10 µM. Apoptosis and G1 arrest in the cells, which were detected by DNA laddering on electrophoresis and flow cytometry, respectively, were observed when the melanoma G-361 cells were treated with Phx-3 at 37°C for 24 h. Concomitantly, the increased melanin formation in G-361 cells was indicated by biochemical and morphological detection of melanin within 24 h exposure to Phx-3. The present results suggest that Phx-3 exclusively demonstrates anti-tumor activity against human malignant melanoma G-361 cells by inducing cell cycle accumulation at G1, differentiation and apoptosis.

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July 2005
Volume 14 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Shimizu S, Suzuki M, Miyazawa K, Yokoyama T, Ohyashiki K, Miyazaki K, Abe A and Tomoda A: Differentiation and apoptosis in human malignant melanoma G-361 cells induced by 2-aminophenoxazine-3-one. Oncol Rep 14: 41-46, 2005
APA
Shimizu, S., Suzuki, M., Miyazawa, K., Yokoyama, T., Ohyashiki, K., Miyazaki, K. ... Tomoda, A. (2005). Differentiation and apoptosis in human malignant melanoma G-361 cells induced by 2-aminophenoxazine-3-one. Oncology Reports, 14, 41-46. https://doi.org/10.3892/or.14.1.41
MLA
Shimizu, S., Suzuki, M., Miyazawa, K., Yokoyama, T., Ohyashiki, K., Miyazaki, K., Abe, A., Tomoda, A."Differentiation and apoptosis in human malignant melanoma G-361 cells induced by 2-aminophenoxazine-3-one". Oncology Reports 14.1 (2005): 41-46.
Chicago
Shimizu, S., Suzuki, M., Miyazawa, K., Yokoyama, T., Ohyashiki, K., Miyazaki, K., Abe, A., Tomoda, A."Differentiation and apoptosis in human malignant melanoma G-361 cells induced by 2-aminophenoxazine-3-one". Oncology Reports 14, no. 1 (2005): 41-46. https://doi.org/10.3892/or.14.1.41