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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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August 2005 Volume 14 Issue 2

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

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Medicine International

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Article

The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo

  • Authors:
    • Masatake Nishi
    • Yoshiyuki Abe
    • Sakashi Fujimori
    • Atsushi Hamamoto
    • Yoshimasa Inoue
    • Noriyuki Miyazaki
    • Yasuhisa Oida
    • Norihiro Ikoma
    • Yasuyuki Ohnishi
    • Hitoshi Yamazaki
    • Yoshito Ueyama
    • Masato Nakamura
  • View Affiliations / Copyright

    Affiliations: Department of Pathology, Tokai University School of Medicine, Bohseidai, Isehara, Kanagawa 259-1193, Japan
  • Pages: 421-424
    |
    Published online on: August 1, 2005
       https://doi.org/10.3892/or.14.2.421
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Abstract

Glutamate cysteine ligase (GCL) plays an important role in the intracellular detoxification of cisplatin (CDDP). GCL is composed of a modifier or light chain subunit (GCLM) and a catalytic or heavy chain subunit (GCLC). Previously, we showed that the GCL subunits enhanced CDDP-resistance in non-small cell lung cancer (NSCLC) xenografts. In small cell lung cancer (SCLC), it is unclear whether the GCL subunits are essential to CDDP-resistance. We examined the gene expression of GCLM and GCLC in four human SCLC xenografts with the real-time polymerase chain reaction (PCR). An in vivo drug sensitivity test with CDDP was performed on the SCLC xenografts. CDDP-resistance was examined as the growth ratio of the relative volume of the treated xenografts to the controls (T/C%). The expression level of GCLM gene in SCLC was significantly lower than that in NSCLC (p=0.0026, Welch's t-test). One of four SCLC xenografts showed 62% of T/C and this was evaluated as CDDP-resistance, while the other three xenografts were sensitive to CDDP in vivo (Mann-Whitney U-test, p<0.01, one-sided). The expression level of the GCLM gene was significantly correlated to T/C% (Fisher's test, p=0.0289, correlations = 0.975), while the GCLC gene expression level was not associated with T/C%. These results suggest that the overexpression of GCLM is correlated with CDDP-resistance in SCLC xenografts in vivo.

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Copy and paste a formatted citation
Spandidos Publications style
Nishi M, Abe Y, Fujimori S, Hamamoto A, Inoue Y, Miyazaki N, Oida Y, Ikoma N, Ohnishi Y, Yamazaki H, Yamazaki H, et al: The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo. Oncol Rep 14: 421-424, 2005.
APA
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N. ... Nakamura, M. (2005). The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo. Oncology Reports, 14, 421-424. https://doi.org/10.3892/or.14.2.421
MLA
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N., Oida, Y., Ikoma, N., Ohnishi, Y., Yamazaki, H., Ueyama, Y., Nakamura, M."The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo". Oncology Reports 14.2 (2005): 421-424.
Chicago
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N., Oida, Y., Ikoma, N., Ohnishi, Y., Yamazaki, H., Ueyama, Y., Nakamura, M."The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo". Oncology Reports 14, no. 2 (2005): 421-424. https://doi.org/10.3892/or.14.2.421
Copy and paste a formatted citation
x
Spandidos Publications style
Nishi M, Abe Y, Fujimori S, Hamamoto A, Inoue Y, Miyazaki N, Oida Y, Ikoma N, Ohnishi Y, Yamazaki H, Yamazaki H, et al: The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo. Oncol Rep 14: 421-424, 2005.
APA
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N. ... Nakamura, M. (2005). The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo. Oncology Reports, 14, 421-424. https://doi.org/10.3892/or.14.2.421
MLA
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N., Oida, Y., Ikoma, N., Ohnishi, Y., Yamazaki, H., Ueyama, Y., Nakamura, M."The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo". Oncology Reports 14.2 (2005): 421-424.
Chicago
Nishi, M., Abe, Y., Fujimori, S., Hamamoto, A., Inoue, Y., Miyazaki, N., Oida, Y., Ikoma, N., Ohnishi, Y., Yamazaki, H., Ueyama, Y., Nakamura, M."The modifier subunit of glutamate cysteine ligase relates to cisplatin resistance in human small cell lung cancer xenografts in vivo". Oncology Reports 14, no. 2 (2005): 421-424. https://doi.org/10.3892/or.14.2.421
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