Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma

  • Authors:
    • Kazutaka Obama
    • Katsuaki Ura
    • Seiji Satoh
    • Yusuke Nakamura
    • Yoichi Furukawa
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/or.14.3.701
  • Pages: 701-706
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Abstract

To disclose molecular mechanisms of cholangiocarcinogenesis and to search for novel diagnostic markers and therapeutic targets for cholangiocarcinoma, we previously analyzed gene-expression profiles of 25 intrahepatic cholangiocarcinomas (ICCs) by means of a cDNA microarray re-presenting 27,648 genes. Among the genes frequently up-regulated in the cancer cells, we focused on PSF2 (partner of SLD five 2), a component of the GINS multiprotein complex that plays a crucial role in initiation of DNA replication. Semi-quantitative RT-PCR analysis of clinical samples confirmed high levels of PSF2 expression in the cancer cells, but expression of this gene was barely detectable in normal vital organs. Transfection of ETK-1 and HuH28 cells with short-interfering RNA specific to PSF2 reduced the amount of transcript and suppressed cell growth, suggesting that PSF2 may play an important role in development of cholangio-carcinoma. The findings reported here provide new insights into human cholangiocarcinogenesis and may contribute to the development of novel therapeutic drugs for this type of liver cancer.

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September 2005
Volume 14 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Obama K, Ura K, Satoh S, Nakamura Y and Furukawa Y: Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma. Oncol Rep 14: 701-706, 2005
APA
Obama, K., Ura, K., Satoh, S., Nakamura, Y., & Furukawa, Y. (2005). Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma. Oncology Reports, 14, 701-706. https://doi.org/10.3892/or.14.3.701
MLA
Obama, K., Ura, K., Satoh, S., Nakamura, Y., Furukawa, Y."Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma". Oncology Reports 14.3 (2005): 701-706.
Chicago
Obama, K., Ura, K., Satoh, S., Nakamura, Y., Furukawa, Y."Up-regulation of PSF2, a member of the GINS multiprotein complex, in intrahepatic cholangiocarcinoma". Oncology Reports 14, no. 3 (2005): 701-706. https://doi.org/10.3892/or.14.3.701