Effect of Notch ligands on in vitro sensitivity to chemo-therapeutic drugs in leukemia and lymphoma cells

  • Authors:
    • Hanae Kogoshi
    • Shuji Tohda
    • Lu Fu
    • Takatoshi Koyama
    • Nobuo Nara
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/or.14.3.723
  • Pages: 723-726
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Abstract

The effect of the recombinant Notch ligand proteins Jagged1 and Delta1 on drug-sensitivity of leukemia and lymphoma cells was examined. Four acute myeloid leukemia (AML) cell lines were cultured in ligand-coated wells and control wells, with cytosine arabinoside (Ara-C), doxorubicin, or mitoxantrone, and nine lymphoid leukemia or lymphoma cell lines were cultured with dexamethasone. The growth was evaluated with a colorimetric assay. The drug-induced growth suppression was slightly reduced by the ligand stimulation in 4 out of 17 combinations of cells versus drugs, such as NB4 cells versus Ara-C. In the remainder, the ligands did not significantly affect the drug-sensitivity. To investigate the possible molecular mechanism of the protective effect, the influence of Jagged1 on Ara-C-induced activation of caspase-3 and PARP, and dephosphorylation of NF-κB was examined in NB4 cells. However, Jagged1 did not obviously affect the activation and dephosphorylation. It is known that stromal cells reduce drug-induced cytotoxicity of leukemia cells. According to our results, the role of Notch ligands in the stroma-mediated resistance seems to be minor and occurs only in a limited context. However, the ligand-coated culture-plates are more similar to the microenvironment in human bone marrow than ordinary plates. We will further examine the clinical usefulness of the drug-sensitivity test using the ligand-coated plates.

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September 2005
Volume 14 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kogoshi H, Tohda S, Fu L, Koyama T and Nara N: Effect of Notch ligands on in vitro sensitivity to chemo-therapeutic drugs in leukemia and lymphoma cells. Oncol Rep 14: 723-726, 2005
APA
Kogoshi, H., Tohda, S., Fu, L., Koyama, T., & Nara, N. (2005). Effect of Notch ligands on in vitro sensitivity to chemo-therapeutic drugs in leukemia and lymphoma cells. Oncology Reports, 14, 723-726. https://doi.org/10.3892/or.14.3.723
MLA
Kogoshi, H., Tohda, S., Fu, L., Koyama, T., Nara, N."Effect of Notch ligands on in vitro sensitivity to chemo-therapeutic drugs in leukemia and lymphoma cells". Oncology Reports 14.3 (2005): 723-726.
Chicago
Kogoshi, H., Tohda, S., Fu, L., Koyama, T., Nara, N."Effect of Notch ligands on in vitro sensitivity to chemo-therapeutic drugs in leukemia and lymphoma cells". Oncology Reports 14, no. 3 (2005): 723-726. https://doi.org/10.3892/or.14.3.723