Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines

  • Authors:
    • Krishan Kumar
    • Qamar Rahman
    • Holger Schipper
    • Claudia Matschegewski
    • Dietmar Schiffmann
    • Thilo Papp
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/or.14.3.743
  • Pages: 743-750
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Abstract

Seven tumour suppressor genes (Chk1, Chk2, Apaf1, Rb1, p53, p16INK4a and p14ARF) and two oncogenes (N-ras and BRAF) were screened in nine human malignant melanoma (HMM) cell lines for point mutations or small deletions/insertions by DGGE, TGGE and SCCP analysis. For the first time in human mesothelioma, Chk1 gene mutations were detected in two of the nine investigated HMM cell lines. P53 gene mutations were found in three cell lines and p16INK4a mutations in 5. Mutation of the Chk1 gene implies a novel disruption mechanism of the p53 pathway in HMM, without affecting p53 itself. According to our knowledge, this is the first mutation screening of Chk1, Chk2, Apaf1 and Rb1 in human malignant mesothelioma.

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September 2005
Volume 14 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Kumar K, Rahman Q, Schipper H, Matschegewski C, Schiffmann D and Papp T: Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines. Oncol Rep 14: 743-750, 2005
APA
Kumar, K., Rahman, Q., Schipper, H., Matschegewski, C., Schiffmann, D., & Papp, T. (2005). Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines. Oncology Reports, 14, 743-750. https://doi.org/10.3892/or.14.3.743
MLA
Kumar, K., Rahman, Q., Schipper, H., Matschegewski, C., Schiffmann, D., Papp, T."Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines". Oncology Reports 14.3 (2005): 743-750.
Chicago
Kumar, K., Rahman, Q., Schipper, H., Matschegewski, C., Schiffmann, D., Papp, T."Mutational analysis of 9 different tumour-associated genes in human malignant mesothelioma cell lines". Oncology Reports 14, no. 3 (2005): 743-750. https://doi.org/10.3892/or.14.3.743