Glycine- and proline-rich glycoprotein isolated from Solanum nigrum Linne activates caspase-3 through cytochrome c in HT-29 cells

  • Authors:
    • Sei-Jung Lee
    • Jeong-Hyeon Ko
    • Kye-Taek Lim
  • View Affiliations

  • Published online on: September 1, 2005     https://doi.org/10.3892/or.14.3.789
  • Pages: 789-796
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Abstract

This study was carried out to investigate apoptotic effects of the glycoprotein (SNL glycoprotein, 150 kDa) isolated from Solanum nigrum Linne, which has been used as an anti-pyretic and anti-cancer agent in folk medicine. We found that the SNL glycoprotein consists of carbohydrate (69.74%) and protein content (30.26%), which has >50% hydrophobic amino acids containing glycine and proline. LDH assay indicated that the SNL glycoprotein has obvious cytotoxic and apoptotic effects (>50% cell death) at 40 µg/ml SNL glycoprotein for 2 h in HT-29 cells. The results showed that the SNL glycoprotein has a stimulatory effect on the release of mitochondrial cytochrome c, cleavages of pro-caspase-9, pro-caspase-3, and poly(ADP-ribose) polymerase (PARP) proteins in HT-29 cells. However, the SNL glycoprotein did not significantly stimulate or change the levels of intracellular reactive oxygen species (ROS). The results of this experiment suggest that the SNL glycoprotein activates caspase-3 in HT-29 cells, independent of ROS.

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September 2005
Volume 14 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lee S, Ko J and Lim K: Glycine- and proline-rich glycoprotein isolated from Solanum nigrum Linne activates caspase-3 through cytochrome c in HT-29 cells. Oncol Rep 14: 789-796, 2005
APA
Lee, S., Ko, J., & Lim, K. (2005). Glycine- and proline-rich glycoprotein isolated from Solanum nigrum Linne activates caspase-3 through cytochrome c in HT-29 cells. Oncology Reports, 14, 789-796. https://doi.org/10.3892/or.14.3.789
MLA
Lee, S., Ko, J., Lim, K."Glycine- and proline-rich glycoprotein isolated from Solanum nigrum Linne activates caspase-3 through cytochrome c in HT-29 cells". Oncology Reports 14.3 (2005): 789-796.
Chicago
Lee, S., Ko, J., Lim, K."Glycine- and proline-rich glycoprotein isolated from Solanum nigrum Linne activates caspase-3 through cytochrome c in HT-29 cells". Oncology Reports 14, no. 3 (2005): 789-796. https://doi.org/10.3892/or.14.3.789