Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer

  • Authors:
    • Takaaki Sugimoto
    • Yuichi Takiguchi
    • Katsushi Kurosu
    • Yasunori Kasahara
    • Nobuhiro Tanabe
    • Koichiro Tatsumi
    • Kenzo Hiroshima
    • Masako Minamihisamatsu
    • Tadaaki Miyamoto
    • Takayuki Kuriyama
  • View Affiliations

  • Published online on: October 1, 2005     https://doi.org/10.3892/or.14.4.823
  • Pages: 823-830
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Malignant tumors induce development of their own stromal tissues during the processes of growth, progression and metastasis. Since the vascular architecture among the various stromal elements is well known to facilitate tumor growth and has been a target of therapy, the importance of stromal fibroblasts has recently been established. To elucidate the interaction between the tumor and its stromal fibroblasts, the present study took advantage of a unique experimental model consisting of a human small-cell lung cancer cell line, WA-ht, and its mouse stromal fibroblast cell line, WA-mFib, both originally derived from a xenograft tumor in a mouse subcutis. Co-culture with the WA-mFib cells significantly augmented the plating efficiency of WA-hT cells in vitro, and their co-inoculation in nude mice shortened latency and tumor doubling time. Histochemical detection of β-gal, transfected into WA-mFib cells, demonstrated their contribution to the nude mouse xenograft tumor formation as its tumor stroma. Elevated hepatocyte growth factor (HGF) from fibroblasts followed by elevated production of vascular endothelial growth factor (VEGF) from both tumor cells and fibroblasts were demonstrated by ELISA in supernatants of their co-culture, accompanied by enhanced colonogenicity of the tumor cells; these enhanced features were not observed in their respective monocultures. Antisense oligonucleotides to HGF cancelled these augmentation effects with co-culture. The findings highlight the substantial roles of tumor stromal fibroblasts, interacting with soluble growth factors, in promoting the malignant propensity of the tumor.

Related Articles

Journal Cover

October 2005
Volume 14 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Sugimoto T, Takiguchi Y, Kurosu K, Kasahara Y, Tanabe N, Tatsumi K, Hiroshima K, Minamihisamatsu M, Miyamoto T, Kuriyama T, Kuriyama T, et al: Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer. Oncol Rep 14: 823-830, 2005
APA
Sugimoto, T., Takiguchi, Y., Kurosu, K., Kasahara, Y., Tanabe, N., Tatsumi, K. ... Kuriyama, T. (2005). Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer. Oncology Reports, 14, 823-830. https://doi.org/10.3892/or.14.4.823
MLA
Sugimoto, T., Takiguchi, Y., Kurosu, K., Kasahara, Y., Tanabe, N., Tatsumi, K., Hiroshima, K., Minamihisamatsu, M., Miyamoto, T., Kuriyama, T."Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer". Oncology Reports 14.4 (2005): 823-830.
Chicago
Sugimoto, T., Takiguchi, Y., Kurosu, K., Kasahara, Y., Tanabe, N., Tatsumi, K., Hiroshima, K., Minamihisamatsu, M., Miyamoto, T., Kuriyama, T."Growth factor-mediated interaction between tumor cells and stromal fibroblasts in an experimental model of human small-cell lung cancer". Oncology Reports 14, no. 4 (2005): 823-830. https://doi.org/10.3892/or.14.4.823