Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line, H-1R

  • Authors:
    • Megumi Nakamura
    • Ken Nakatani
    • Katsuhiro Uzawa
    • Kanae Ono
    • Hisako Uesugi
    • Katsunori Ogawara
    • Masashi Shiiba
    • Hiroki Bukawa
    • Hidetaka Yokoe
    • Takeshi Wada
    • Shigeyuki Fujita
    • Hideki Tanzawa
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/or.14.5.1281
  • Pages: 1281-1286
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Abstract

Cisplatin (CDDP) is a widely used potent chemotherapeutic agent for many malignancies. However, the mechanism of resistance to CDDP remains unclear. To investigate the molecular mechanism, we established a CDDP-resistant cell line (H-1R) from a CDDP-sensitive cell line (H-1) which was derived from moderately differentiated squamous cell carcinoma of the lower gingiva. The 3-(3,4-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT) assay indicated that H-1R had a 10-fold greater resistance to CDDP than H-1. When we compared gene expression levels in the cell lines using an in-house cDNA microarray, which represented 2,201 genes originating from normal oral tissue, primary oral cancer, and oral cancer cell lines, 12 genes showing elevated mRNA expression in H-1R compared with H-1 were identified. Among them, the up-regulated expression of ATP-binding cassette transporter genes (MDR1, MRP1, and MRP2), CD55, and PGK1 and down-regulated expression of Caveolin 1 were further confirmed by semiquantitative reverse transcriptase-polymerase chain reaction (PCR) or real-time PCR. Our results suggest that H-1 and H-1R cell lines could be useful for elucidating the candidate genes responsible for CDDP resistance, including the genes found in this study.

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November 2005
Volume 14 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Nakamura M, Nakatani K, Uzawa K, Ono K, Uesugi H, Ogawara K, Shiiba M, Bukawa H, Yokoe H, Wada T, Wada T, et al: Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line, H-1R. Oncol Rep 14: 1281-1286, 2005.
APA
Nakamura, M., Nakatani, K., Uzawa, K., Ono, K., Uesugi, H., Ogawara, K. ... Tanzawa, H. (2005). Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line, H-1R. Oncology Reports, 14, 1281-1286. https://doi.org/10.3892/or.14.5.1281
MLA
Nakamura, M., Nakatani, K., Uzawa, K., Ono, K., Uesugi, H., Ogawara, K., Shiiba, M., Bukawa, H., Yokoe, H., Wada, T., Fujita, S., Tanzawa, H."Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line, H-1R". Oncology Reports 14.5 (2005): 1281-1286.
Chicago
Nakamura, M., Nakatani, K., Uzawa, K., Ono, K., Uesugi, H., Ogawara, K., Shiiba, M., Bukawa, H., Yokoe, H., Wada, T., Fujita, S., Tanzawa, H."Establishment and characterization of a cisplatin-resistant oral squamous cell carcinoma cell line, H-1R". Oncology Reports 14, no. 5 (2005): 1281-1286. https://doi.org/10.3892/or.14.5.1281