Targeted adriamycin delivery to MXT-B2 metastatic mammary carcinoma cells by transferrin liposomes: Effect of adriamycin ADR-to-lipid ratio

  • Authors:
    • Lluis A. Lopez-Barcons
    • Dolores Polo
    • Ana Llorens
    • Francesca Reig
    • Angels Fabra
  • View Affiliations

  • Published online on: November 1, 2005     https://doi.org/10.3892/or.14.5.1337
  • Pages: 1337-1343
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Abstract

In the protein-targeted therapy for cancer, transferrin (Tf) is used to reach a selective and specific target in cancer cells. Tf is used conjugated to chemotherapeutic drugs, insulin, toxins, antibodies, polymers, nanoparticles, lipoplexes and liposomes. Using this latter approach, hydrophobically derivatized Tf was incorporated to liposomal bilayers. The biological activity of Tf-liposome was tested using MXT-B2 cells, a metastatic mammary carcinoma cell line. In Tf binding assays, the Scatchard analysis indicated 4.5x105 Tf receptors/cell. In cell growth assays, Tf-liposomes stimulated cell growth in a dose-dependent manner, up to a maximum of 32% of the total free Tf stimulation. Following this, we prepared Tf-liposomes encapsulating adriamycin (ADR) at two different ADR-to-lipid ratios. In vitro cytotoxicity assays against MXT-B2 cells gave IC50 values 2.1-times lower for Tf-liposomal ADR in comparison to control liposomal ADR. However, similar IC50 values were found for low ADR-to-lipid ratio Tf-liposomal ADR, as well as for control liposomal ADR. The free Tf added in excess increased the IC50 value of Tf-liposomal ADR by 51%, while the IC50 value of control liposomal ADR was unaffected, supporting a receptor-mediated mechanism of targeting by Tf. In addition, the lower IC50 value is correlated with a higher total of ADR accumulation in the cells.

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November 2005
Volume 14 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Lopez-Barcons LA, Polo D, Llorens A, Reig F and Fabra A: Targeted adriamycin delivery to MXT-B2 metastatic mammary carcinoma cells by transferrin liposomes: Effect of adriamycin ADR-to-lipid ratio. Oncol Rep 14: 1337-1343, 2005
APA
Lopez-Barcons, L.A., Polo, D., Llorens, A., Reig, F., & Fabra, A. (2005). Targeted adriamycin delivery to MXT-B2 metastatic mammary carcinoma cells by transferrin liposomes: Effect of adriamycin ADR-to-lipid ratio. Oncology Reports, 14, 1337-1343. https://doi.org/10.3892/or.14.5.1337
MLA
Lopez-Barcons, L. A., Polo, D., Llorens, A., Reig, F., Fabra, A."Targeted adriamycin delivery to MXT-B2 metastatic mammary carcinoma cells by transferrin liposomes: Effect of adriamycin ADR-to-lipid ratio". Oncology Reports 14.5 (2005): 1337-1343.
Chicago
Lopez-Barcons, L. A., Polo, D., Llorens, A., Reig, F., Fabra, A."Targeted adriamycin delivery to MXT-B2 metastatic mammary carcinoma cells by transferrin liposomes: Effect of adriamycin ADR-to-lipid ratio". Oncology Reports 14, no. 5 (2005): 1337-1343. https://doi.org/10.3892/or.14.5.1337