Decrease in size of azoxymethane induced colon carcinoma in F344 rats by 180-day fermented miso

  • Authors:
    • Yoshie Ohuchi
    • Yuki Myojin
    • Fumio Shimamoto
    • Naoki Kashimoto
    • Kenji Kamiya
    • Hiromitsu Watanabe
  • View Affiliations

  • Published online on: December 1, 2005     https://doi.org/10.3892/or.14.6.1559
  • Pages: 1559-1564
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Abstract

The present study was designed to investigate the effects of fermented miso (fermented soybean paste) on the induction of colon tumors by azoxymethane (AOM) in male F344 rats. A total of 91 rats, 6 weeks of age, were divided into 5 groups and given weekly subcutaneous injections of AOM (15 mg/kg body wt) for 3 weeks. The animals were placed on diets one week before the first AOM dose: commercial normal control MF diet or a diet containing 10% 2-year, 180-day fermented, or 3-4-day fermented miso. There were no differences in body and organ weights, and no aberrant crypt foci (ACF) among carcinogen-treated groups at week 25. The rates of tumor incidence were 45%, 85%, 75% and 60% with the 2-year, 180-day, and 3-4-day fermented miso and MF, respectively, and those for colon tumors were 34%, 55%, 60% and 55%, respectively. The size of well-differentiated adenocarcinomas and total (well differentiated and signet ring cell) adenocarcinomas in the 180-day fermented miso group was significantly smaller than that in the 2-year fermented miso and MF+AOM groups. Nuclear staining of β-catenin in colon tumors was increased for the 3-4-day fermented miso compared to the 180-day fermented miso. Cdx2 staining tendency was decreased in colon tumors and adenocarcinomas compared to normal mucosa and ACF, which stained in 100% of cases. In addition, the PCNA index was significantly reduced in the 180-day group compared with those groups receiving the 3-4-day fermented miso and MF diet. The germinal region was also decreased. The present results indicate that dietary supplementation with 180-day fermented dietary miso could act as a chemopreventive agent for colon carcinogenesis.

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December 2005
Volume 14 Issue 6

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ohuchi Y, Myojin Y, Shimamoto F, Kashimoto N, Kamiya K and Watanabe H: Decrease in size of azoxymethane induced colon carcinoma in F344 rats by 180-day fermented miso. Oncol Rep 14: 1559-1564, 2005.
APA
Ohuchi, Y., Myojin, Y., Shimamoto, F., Kashimoto, N., Kamiya, K., & Watanabe, H. (2005). Decrease in size of azoxymethane induced colon carcinoma in F344 rats by 180-day fermented miso. Oncology Reports, 14, 1559-1564. https://doi.org/10.3892/or.14.6.1559
MLA
Ohuchi, Y., Myojin, Y., Shimamoto, F., Kashimoto, N., Kamiya, K., Watanabe, H."Decrease in size of azoxymethane induced colon carcinoma in F344 rats by 180-day fermented miso". Oncology Reports 14.6 (2005): 1559-1564.
Chicago
Ohuchi, Y., Myojin, Y., Shimamoto, F., Kashimoto, N., Kamiya, K., Watanabe, H."Decrease in size of azoxymethane induced colon carcinoma in F344 rats by 180-day fermented miso". Oncology Reports 14, no. 6 (2005): 1559-1564. https://doi.org/10.3892/or.14.6.1559