p53 enhances the Δ-24 conditionally replicative adenovirus anti-glioma effect

  • Authors:
    • P. G. Mitlianga
    • C. Sioka
    • G. Vartholomatos
    • A. Goussia
    • K. Polyzoidis
    • J. S. Rao
    • A. P. Kyritsis
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/or.15.1.149
  • Pages: 149-153
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Abstract

Previous studies have demonstrated that the conditionally replicative adenovirus Ad5Δ24 is a powerful cytolytic agent against glioma selectively affecting cells with a defective p16/Rb/E2F pathway. The p53 protein is also known to be an apoptotic factor for glioma cells. In this study, we examined the simultaneous delivery of the combination of exogenous p53 and Ad5Δ24 adenovirus in glioma cells. Infecting cells with low doses of adenovirus p53 and Ad5Δ24 resulted in an additive effect on cell death. The cell death induced by both agents was independent of the p53 status of cells. Flow cytometry revealed that the potent anti-tumor effect induced by the mixture of Ad5CMV-p53 and Ad5Δ24 adenoviruses was due to a combination of apoptosis and cell lysis. Our results indicate that Ad5CMV-p53 enhances the oncolytic effect of the Ad5Δ24 adenovirus, and the combination of adenovirus Ad5Δ24 and Ad5CMV-p53 may thus be a potential therapeutic tool for gliomas.

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January 2006
Volume 15 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Mitlianga PG, Sioka C, Vartholomatos G, Goussia A, Polyzoidis K, Rao JS and Kyritsis AP: p53 enhances the Δ-24 conditionally replicative adenovirus anti-glioma effect. Oncol Rep 15: 149-153, 2006
APA
Mitlianga, P.G., Sioka, C., Vartholomatos, G., Goussia, A., Polyzoidis, K., Rao, J.S., & Kyritsis, A.P. (2006). p53 enhances the Δ-24 conditionally replicative adenovirus anti-glioma effect. Oncology Reports, 15, 149-153. https://doi.org/10.3892/or.15.1.149
MLA
Mitlianga, P. G., Sioka, C., Vartholomatos, G., Goussia, A., Polyzoidis, K., Rao, J. S., Kyritsis, A. P."p53 enhances the Δ-24 conditionally replicative adenovirus anti-glioma effect". Oncology Reports 15.1 (2006): 149-153.
Chicago
Mitlianga, P. G., Sioka, C., Vartholomatos, G., Goussia, A., Polyzoidis, K., Rao, J. S., Kyritsis, A. P."p53 enhances the Δ-24 conditionally replicative adenovirus anti-glioma effect". Oncology Reports 15, no. 1 (2006): 149-153. https://doi.org/10.3892/or.15.1.149