Real-time RT-PCR analysis of human histidine decarboxylase, a new marker for several types of leukemia and cancer

  • Authors:
    • Esther Melgarejo
    • Miguel Ángel Medina
    • José Carlos Paz
    • Francisca Sánchez-Jiménez
    • José Luis Urdiales
  • View Affiliations

  • Published online on: January 1, 2006     https://doi.org/10.3892/or.15.1.193
  • Pages: 193-197
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Histamine is involved in different physiological and pathological responses, such as immune response, gastric acid secretion or neurotransmission, as either angiogenesis or cancer. Histidine decarboxylase (HDC) catalyzes the formation of histamine from histidine. HDC has been suggested as a new marker for neuroendocrine differentiation, inflammatory pathologies and several leukemia and highly malignant forms of cancer, such as melanoma and small cell lung carcinoma. In the present work, we describe the use of Syber Green-based quantitative real-time RT-PCR to determine the expression of histidine decarboxylase in human cells and tissue. As an internal control, glyceraldehyde 3-phosphate dehydrogenase was also amplified. The linear dynamic range of the assay covered 4 orders of magnitude for HDC amplification. The detection limit was 0.1 ng of total RNA extracted from HMC-1 cells. This method is simple, rapid, sensitive, and quantitative, and allows for the specific identification of cells and tissue expressing HDC, stressing its potential diagnostic usefulness in malignancies in which HDC is described as a new marker.

Related Articles

Journal Cover

January 2006
Volume 15 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Melgarejo E, Medina MÁ, Paz JC, Sánchez-Jiménez F and Urdiales JL: Real-time RT-PCR analysis of human histidine decarboxylase, a new marker for several types of leukemia and cancer. Oncol Rep 15: 193-197, 2006
APA
Melgarejo, E., Medina, M.Á., Paz, J.C., Sánchez-Jiménez, F., & Urdiales, J.L. (2006). Real-time RT-PCR analysis of human histidine decarboxylase, a new marker for several types of leukemia and cancer. Oncology Reports, 15, 193-197. https://doi.org/10.3892/or.15.1.193
MLA
Melgarejo, E., Medina, M. Á., Paz, J. C., Sánchez-Jiménez, F., Urdiales, J. L."Real-time RT-PCR analysis of human histidine decarboxylase, a new marker for several types of leukemia and cancer". Oncology Reports 15.1 (2006): 193-197.
Chicago
Melgarejo, E., Medina, M. Á., Paz, J. C., Sánchez-Jiménez, F., Urdiales, J. L."Real-time RT-PCR analysis of human histidine decarboxylase, a new marker for several types of leukemia and cancer". Oncology Reports 15, no. 1 (2006): 193-197. https://doi.org/10.3892/or.15.1.193