Prostate-specific antigen-reactive cytotoxic T lymphocyte precursors in colon cancer patients

  • Authors:
    • Yi Wang
    • Mamoru Harada
    • Hirohisa Yano
    • Sachiko Ogasawara
    • Masahiro Tanaka
    • Akira Yamada
    • Kyogo Itoh
  • View Affiliations

  • Published online on: February 1, 2006     https://doi.org/10.3892/or.15.2.317
  • Pages: 317-321
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Prostate-specific antigen (PSA) is a representative of the prostate-related antigens, and has been considered to be a tumor marker of prostate cancer. However, some studies suggest that PSA could be produced by several types of tumors. In the present study, we attempted to determine whether or not PSA could be a target molecule in specific immunotherapy for patients with colon cancer. Five colon cancer cell lines were examined for their PSA expression at the mRNA and protein levels by RT-PCR and immunocytostaining, respectively. As a result, four cell lines were found to be positive for PSA at both the mRNA and protein levels. We also attempted to determine whether PSA-reactive cytotoxic T lymphocytes (CTLs) could be induced from the peripheral blood mononuclear cells (PBMCs) of HLA-A24+ colon cancer patients by in vitro stimulation with PSA-derived peptides. As a consequence, PSA peptide-specific CTLs could be generated from the PBMCs of male and female colon cancer patients. Their cytotoxicity against HLA-A24+ PSA-expressing colon cancer cells was dependent on HLA class I-restricted and CD8+ T cells. These findings indicate that PSA-reactive CTL precursors are present in the periphery of colon cancer patients, and that PSA could be a target molecule in specific immunotherapy to colon cancer.

Related Articles

Journal Cover

February 2006
Volume 15 Issue 2

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Wang Y, Harada M, Yano H, Ogasawara S, Tanaka M, Yamada A and Itoh K: Prostate-specific antigen-reactive cytotoxic T lymphocyte precursors in colon cancer patients. Oncol Rep 15: 317-321, 2006.
APA
Wang, Y., Harada, M., Yano, H., Ogasawara, S., Tanaka, M., Yamada, A., & Itoh, K. (2006). Prostate-specific antigen-reactive cytotoxic T lymphocyte precursors in colon cancer patients. Oncology Reports, 15, 317-321. https://doi.org/10.3892/or.15.2.317
MLA
Wang, Y., Harada, M., Yano, H., Ogasawara, S., Tanaka, M., Yamada, A., Itoh, K."Prostate-specific antigen-reactive cytotoxic T lymphocyte precursors in colon cancer patients". Oncology Reports 15.2 (2006): 317-321.
Chicago
Wang, Y., Harada, M., Yano, H., Ogasawara, S., Tanaka, M., Yamada, A., Itoh, K."Prostate-specific antigen-reactive cytotoxic T lymphocyte precursors in colon cancer patients". Oncology Reports 15, no. 2 (2006): 317-321. https://doi.org/10.3892/or.15.2.317