Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer

  • Authors:
    • Jinyu Gu
    • Hirofumi Yamamoto
    • Minoru Ogawa
    • Chew Yee Ngan
    • Katsuki Danno
    • Hideyuki Hemmi
    • Naganori Kyo
    • Ichiro Takemasa
    • Masataka Ikeda
    • Mitsugu Sekimoto
    • Morito Monden
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/or.15.4.779
  • Pages: 779-783
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Abstract

Angiogenesis is a compensatory mechanism that enables malignant tumors to survive in an oxygen-deficient environment. To test our hypothesis that hypoxia stimulates the production of angiopoietin-2 (Ang-2) in colorectal cancer (CRC), we investigated the expression of Ang-2 in three cultured CRC cell lines, and in specimens from 11 CRC metastatic liver tumors. Hypoxia-induced Ang-2 mRNA expression was clearly evident in HCT116 cells that did not express Ang-2 under normoxic conditions. Ang-2 mRNA was detected only after 48 h in hypoxic serum-deprived cultures in a LoVo cell line, and under both normoxic and hypoxic conditions without any noticeable difference in the HT29 cells. There was a stepwise increase in Ang-2 expression from the periphery to the central part of the liver metastatic foci, whereas an inverse result was noted in tumor blood vessels, with a gradual decrease in CD31-positive ECs from the edge to the central region of the metastatic lesion. An expression pattern similar to Ang-2 was found in glucose transporter 1 (Glut-1), a known hypoxia-induced factor. These findings suggest that hypoxia plays an important role in inducing the expression of Ang-2 in CRC.

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April 2006
Volume 15 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Gu J, Yamamoto H, Ogawa M, Ngan CY, Danno K, Hemmi H, Kyo N, Takemasa I, Ikeda M, Sekimoto M, Sekimoto M, et al: Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer. Oncol Rep 15: 779-783, 2006
APA
Gu, J., Yamamoto, H., Ogawa, M., Ngan, C.Y., Danno, K., Hemmi, H. ... Monden, M. (2006). Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer. Oncology Reports, 15, 779-783. https://doi.org/10.3892/or.15.4.779
MLA
Gu, J., Yamamoto, H., Ogawa, M., Ngan, C. Y., Danno, K., Hemmi, H., Kyo, N., Takemasa, I., Ikeda, M., Sekimoto, M., Monden, M."Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer". Oncology Reports 15.4 (2006): 779-783.
Chicago
Gu, J., Yamamoto, H., Ogawa, M., Ngan, C. Y., Danno, K., Hemmi, H., Kyo, N., Takemasa, I., Ikeda, M., Sekimoto, M., Monden, M."Hypoxia-induced up-regulation of angiopoietin-2 in colorectal cancer". Oncology Reports 15, no. 4 (2006): 779-783. https://doi.org/10.3892/or.15.4.779