Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection

  • Authors:
    • Shiori Maenaka
    • Tetsurou Hikichi
    • Miwa A. Imai
    • Toshinari Minamoto
    • Ei Kawahara
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/or.15.4.791
  • Pages: 791-795
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Abstract

To ascertain the implications of loss of imprinting (LOI) of the insulin-like growth factor II gene (IGF2) for carcinogenesis, the precise frequency of LOI in colorectal carcinoma was examined using a laser capture microdissection method, and compared to the matched normal colorectal mucosa. LOI was examined by PCR-restriction fragment length polymorphism in combination with direct sequencing. The status was assigned as imprinting when PCR-RFLP showed only one band or sequence with a single peak, otherwise cases were classified as LOI. LOI was found in 13 of 24 informative cases of carcinoma (54%), which was higher than the ratios reported previously. LOI was also found in the normal colorectal mucosae in 14 cases (58%). The LOIs in carcinomas and in the normal mucosae were closely correlated: 10 of 13 LOI-positive carcinomas showed LOI in the matched normal mucosae. These results suggest that LOI of IGF2 in colorectal carcinoma and LOI in the background mucosa play important roles in carcinogenesis.

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April 2006
Volume 15 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Maenaka S, Hikichi T, Imai MA, Minamoto T and Kawahara E: Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection. Oncol Rep 15: 791-795, 2006
APA
Maenaka, S., Hikichi, T., Imai, M.A., Minamoto, T., & Kawahara, E. (2006). Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection. Oncology Reports, 15, 791-795. https://doi.org/10.3892/or.15.4.791
MLA
Maenaka, S., Hikichi, T., Imai, M. A., Minamoto, T., Kawahara, E."Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection". Oncology Reports 15.4 (2006): 791-795.
Chicago
Maenaka, S., Hikichi, T., Imai, M. A., Minamoto, T., Kawahara, E."Loss of imprinting in IGF2 in colorectal carcinoma assessed by microdissection". Oncology Reports 15, no. 4 (2006): 791-795. https://doi.org/10.3892/or.15.4.791