Enhanced in vitro invasiveness of ovarian cancer cells through up-regulation of VEGF and induction of MMP-2

  • Authors:
    • Ali Zhang
    • Li Meng
    • Qunsheng Wang
    • Ling Xi
    • Gang Chen
    • Shixuan Wang
    • Jianfeng Zhou
    • Yunping Lu
    • Ding Ma
  • View Affiliations

  • Published online on: April 1, 2006     https://doi.org/10.3892/or.15.4.831
  • Pages: 831-836
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Vascular endothelial growth factor (VEGF) has been identified to be important in tumor angiogenesis, which is essential for the growth, invasion, and metastasis of solid tumors. The aim of this study was to determine the effect of VEGF overexpression on the invasion of human epithelial ovarian cancer cells in vitro and the possible mechanism involved. The VEGF165 cDNA was transfected into ovarian tumor cell lines CAOV3 and COC1 to promote the expression of VEGF. The VEGF expression and matrix metalloproteinase (MMP)-2 activity were examined by RT-PCR, Western blot analysis and gelatin zymography. A modified Boyden chamber assay was used to test tumor cell invasion in vitro. All cells overexpressing VEGF displayed an enhanced in vitro invasiveness through Matrigel-coated filters with Boyden chamber invasion assay. MMP-2 mRNA and protein were significantly increased during VEGF165 cDNA transfection; MMP-2 activity was also increased. The invasion property of ovarian cancer cells was abrogated with VEGF neutralizing antibody. Our data indicated that the expression of VEGF gave impetus to the in vitro invasion of ovarian cancer cells by stimulating the production and functional activities of MMP-2, which may be a key component of VEGF in promoting ovarian cancer cell invasion. VEGF may constitute a novel therapeutic target for antiangiogenic cancer therapy.

Related Articles

Journal Cover

April 2006
Volume 15 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Zhang A, Meng L, Wang Q, Xi L, Chen G, Wang S, Zhou J, Lu Y and Ma D: Enhanced in vitro invasiveness of ovarian cancer cells through up-regulation of VEGF and induction of MMP-2. Oncol Rep 15: 831-836, 2006
APA
Zhang, A., Meng, L., Wang, Q., Xi, L., Chen, G., Wang, S. ... Ma, D. (2006). Enhanced in vitro invasiveness of ovarian cancer cells through up-regulation of VEGF and induction of MMP-2. Oncology Reports, 15, 831-836. https://doi.org/10.3892/or.15.4.831
MLA
Zhang, A., Meng, L., Wang, Q., Xi, L., Chen, G., Wang, S., Zhou, J., Lu, Y., Ma, D."Enhanced in vitro invasiveness of ovarian cancer cells through up-regulation of VEGF and induction of MMP-2". Oncology Reports 15.4 (2006): 831-836.
Chicago
Zhang, A., Meng, L., Wang, Q., Xi, L., Chen, G., Wang, S., Zhou, J., Lu, Y., Ma, D."Enhanced in vitro invasiveness of ovarian cancer cells through up-regulation of VEGF and induction of MMP-2". Oncology Reports 15, no. 4 (2006): 831-836. https://doi.org/10.3892/or.15.4.831