Differential expression of alternatively spliced mRNA forms of the insulin-like growth factor 1 receptor in human neuroendocrine tumors

  • Authors:
    • Lorenza Vitale
    • Luca Lenzi
    • Shane A. Huntsman
    • Silvia Canaider
    • Flavia Frabetti
    • Raffaella Casadei
    • Federica Facchin
    • Paolo Carinci
    • Maria Zannotti
    • Domenico Coppola
    • Pierluigi Strippoli
  • View Affiliations

  • Published online on: May 1, 2006     https://doi.org/10.3892/or.15.5.1249
  • Pages: 1249-1256
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Abstract

The activation of the insulin-like growth factor 1/IGF1 receptor system (IGF1/IGF1R) is a critical event in the transformation and tumorigenicity processes in a wide variety of human tumors. The IGF1/IGF1R system has been recently studied in carcinoid tumors that often arise in the gastrointestinal tract; these tumors are characterized by hypersecretion of bioamines and neuropeptides, leading to functional tumor disease. Two alternatively spliced IGF1R mRNA transcripts have been described to differ by only three nucleotides (CAG) in the coding sequence, resulting in an amino-acid change from the originally described Thr-Gly to an Arg in the extracellular portion of the receptor β subunit. In transfected Chinese hamster ovary cells, the form without CAG (CAG−) exhibited an approximate 2-fold increase in IGF1 stimulation of activities required for its mitogenic properties. In this study, we examine the relative expression of the two IGF1R mRNA isoforms by a semiquantitative RT-PCR approach using highly standardized conditions, β-2 microglobulin (B2M) as a reference gene and gel imaging analysis. We analyzed a large series of human neuroendocrine tumors (32 samples) and 9 normal tissues. A significant higher expression of both isoforms in the tumor samples (≈2-fold increase) was found, while a constant CAG+/CAG− IGF1R mRNA isoforms of an approximate 3:1 ratio was observed in all tumoral and normal cell types studied. The phylogenetic study of the IGF1R locus in several species suggests that human IGF1R CAG− mRNA isoform is evolutionarily more recent compared to the IGF1R CAG+ mRNA isoform and it could be used by the splicing apparatus at this intron/exon junction with a lower efficiency. This study highlights the relevance of IGF1R mRNA expression in neuroendocrine tumor cells, and the constant presence of ‘subtle’ alternative splicing for the IGF1R locus.

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May 2006
Volume 15 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Vitale L, Lenzi L, Huntsman SA, Canaider S, Frabetti F, Casadei R, Facchin F, Carinci P, Zannotti M, Coppola D, Coppola D, et al: Differential expression of alternatively spliced mRNA forms of the insulin-like growth factor 1 receptor in human neuroendocrine tumors. Oncol Rep 15: 1249-1256, 2006
APA
Vitale, L., Lenzi, L., Huntsman, S.A., Canaider, S., Frabetti, F., Casadei, R. ... Strippoli, P. (2006). Differential expression of alternatively spliced mRNA forms of the insulin-like growth factor 1 receptor in human neuroendocrine tumors. Oncology Reports, 15, 1249-1256. https://doi.org/10.3892/or.15.5.1249
MLA
Vitale, L., Lenzi, L., Huntsman, S. A., Canaider, S., Frabetti, F., Casadei, R., Facchin, F., Carinci, P., Zannotti, M., Coppola, D., Strippoli, P."Differential expression of alternatively spliced mRNA forms of the insulin-like growth factor 1 receptor in human neuroendocrine tumors". Oncology Reports 15.5 (2006): 1249-1256.
Chicago
Vitale, L., Lenzi, L., Huntsman, S. A., Canaider, S., Frabetti, F., Casadei, R., Facchin, F., Carinci, P., Zannotti, M., Coppola, D., Strippoli, P."Differential expression of alternatively spliced mRNA forms of the insulin-like growth factor 1 receptor in human neuroendocrine tumors". Oncology Reports 15, no. 5 (2006): 1249-1256. https://doi.org/10.3892/or.15.5.1249