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Oncology Reports
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Print ISSN: 1021-335X Online ISSN: 1791-2431
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June 2006 Volume 15 Issue 6

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Journals

International Journal of Molecular Medicine

International Journal of Molecular Medicine

International Journal of Molecular Medicine is an international journal devoted to molecular mechanisms of human disease.

International Journal of Oncology

International Journal of Oncology

International Journal of Oncology is an international journal devoted to oncology research and cancer treatment.

Molecular Medicine Reports

Molecular Medicine Reports

Covers molecular medicine topics such as pharmacology, pathology, genetics, neuroscience, infectious diseases, molecular cardiology, and molecular surgery.

Oncology Reports

Oncology Reports

Oncology Reports is an international journal devoted to fundamental and applied research in Oncology.

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine

Experimental and Therapeutic Medicine is an international journal devoted to laboratory and clinical medicine.

Oncology Letters

Oncology Letters

Oncology Letters is an international journal devoted to Experimental and Clinical Oncology.

Biomedical Reports

Biomedical Reports

Explores a wide range of biological and medical fields, including pharmacology, genetics, microbiology, neuroscience, and molecular cardiology.

Molecular and Clinical Oncology

Molecular and Clinical Oncology

International journal addressing all aspects of oncology research, from tumorigenesis and oncogenes to chemotherapy and metastasis.

World Academy of Sciences Journal

World Academy of Sciences Journal

Multidisciplinary open-access journal spanning biochemistry, genetics, neuroscience, environmental health, and synthetic biology.

International Journal of Functional Nutrition

International Journal of Functional Nutrition

Open-access journal combining biochemistry, pharmacology, immunology, and genetics to advance health through functional nutrition.

International Journal of Epigenetics

International Journal of Epigenetics

Publishes open-access research on using epigenetics to advance understanding and treatment of human disease.

Medicine International

Medicine International

An International Open Access Journal Devoted to General Medicine.

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June 2006 Volume 15 Issue 6

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Article

A useful protocol for analyses of mutations of the epidermal growth factor receptor gene

  • Authors:
    • Dage Liu
    • Jun Nakano
    • Masaki Ueno
    • Daiki Masuya
    • Takashi Nakashima
    • Hiroyasu Yokomise
    • Kouichi Yube
    • Cheng-Long Huang

  • View Affiliations / Copyright

    Affiliations: Second Department of Surgery, Kagawa University, 1750-1, Miki-cho, Kita-gun, Kagawa 761-0793, Japan
  • Pages: 1503-1505
    |
    Published online on: June 1, 2006
       https://doi.org/10.3892/or.15.6.1503
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Abstract

Recent studies have reported that mutations of the epidermal growth factor receptor (EGFR) gene are associated with the responsiveness of tyrosine kinase inhibitors (TKIs), which are molecular targets for non-small cell lung cancer (NSCLC). To provide genetic analyses for NSCLC patients, a simple and reliable method using paraffin-embedded materials is needed. The DEXPAT DNA extraction kit was used for DNA extraction from paraffin-embedded materials. DNA was amplified using the nested PCR technique, then analyzed by direct sequencing for EGFR mutations (exons 18 to 21). The phenol/chloroform extraction for DNA was also performed for comparison. When the DEXPAT kit was used, distinct bands were observed in all products after nested PCR assays of paraffin-embedded materials. Distinct sequencing signals were obtained. Results from the sequencing analysis of paraffin-embedded materials and frozen materials were completely concordant. The current study suggests that DNA extraction with the DEXPAT kit followed by nested PCR is a simple and reliable technique for analyzing the EGFR gene status with paraffin-embedded samples.

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Copy and paste a formatted citation
Spandidos Publications style
Liu D, Nakano J, Ueno M, Masuya D, Nakashima T, Yokomise H, Yube K and Huang C: A useful protocol for analyses of mutations of the epidermal growth factor receptor gene. Oncol Rep 15: 1503-1505, 2006.
APA
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H. ... Huang, C. (2006). A useful protocol for analyses of mutations of the epidermal growth factor receptor gene. Oncology Reports, 15, 1503-1505. https://doi.org/10.3892/or.15.6.1503
MLA
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H., Yube, K., Huang, C."A useful protocol for analyses of mutations of the epidermal growth factor receptor gene". Oncology Reports 15.6 (2006): 1503-1505.
Chicago
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H., Yube, K., Huang, C."A useful protocol for analyses of mutations of the epidermal growth factor receptor gene". Oncology Reports 15, no. 6 (2006): 1503-1505. https://doi.org/10.3892/or.15.6.1503
Copy and paste a formatted citation
x
Spandidos Publications style
Liu D, Nakano J, Ueno M, Masuya D, Nakashima T, Yokomise H, Yube K and Huang C: A useful protocol for analyses of mutations of the epidermal growth factor receptor gene. Oncol Rep 15: 1503-1505, 2006.
APA
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H. ... Huang, C. (2006). A useful protocol for analyses of mutations of the epidermal growth factor receptor gene. Oncology Reports, 15, 1503-1505. https://doi.org/10.3892/or.15.6.1503
MLA
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H., Yube, K., Huang, C."A useful protocol for analyses of mutations of the epidermal growth factor receptor gene". Oncology Reports 15.6 (2006): 1503-1505.
Chicago
Liu, D., Nakano, J., Ueno, M., Masuya, D., Nakashima, T., Yokomise, H., Yube, K., Huang, C."A useful protocol for analyses of mutations of the epidermal growth factor receptor gene". Oncology Reports 15, no. 6 (2006): 1503-1505. https://doi.org/10.3892/or.15.6.1503
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