Detection and expression of human papillomavirus oncogenes in non-small cell lung cancer

  • Authors:
    • Marco Ciotti
    • Laura Giuliani
    • Vincenzo Ambrogi
    • Corrado Ronci
    • Arrigo Benedetto
    • Tommaso C. Mineo
    • Kari Syrjänen
    • Cartesio Favalli
  • View Affiliations

  • Published online on: July 1, 2006     https://doi.org/10.3892/or.16.1.183
  • Pages: 183-189
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Abstract

Human papillomavirus (HPV) has been found in lung cancer cases with variable frequency. In the present study, we analysed a series of 38 patients with non-small cell lung cancer (NSCLC) (21 paraffin-embedded archival samples and 17 fresh surgical specimens) for the presence of E6 and E7 oncogenes of HPV16, 18 and 31. Eight of the tumours were positive (21%): six HPV16, one HPV16+18, and one HPV31. The normal tissue surrounding the HPV-positive tumour was negative for the presence of the virus. Sequencing analysis of URR, of HPV16, which was the most frequently found HPV type in our cases, showed an adenosine deletion at nucleotide 7861 (E2-binding site) in four out of six patients. Sequencing of the entire E6 and E7 genes of HPV16 showed a T to G transition at nucleotide position 350 of E6, in all examined cases. This mutation is associated to the European variant of HPV16. Analysis of E6 and E7 transcripts was performed on the six fresh surgical specimens infected by HPV16. Our study showed that all of the tumours investigated, except one, contained E6 and E7 transcripts. Only in one case could we identify an unspliced form of the E6 transcript. Our results strengthen the relationship between HPV and NSCLC and support the hypothesis that HPV infection could play a role in bronchial carcinogenesis.

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July 2006
Volume 16 Issue 1

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Ciotti M, Giuliani L, Ambrogi V, Ronci C, Benedetto A, Mineo TC, Syrjänen K and Favalli C: Detection and expression of human papillomavirus oncogenes in non-small cell lung cancer. Oncol Rep 16: 183-189, 2006
APA
Ciotti, M., Giuliani, L., Ambrogi, V., Ronci, C., Benedetto, A., Mineo, T.C. ... Favalli, C. (2006). Detection and expression of human papillomavirus oncogenes in non-small cell lung cancer. Oncology Reports, 16, 183-189. https://doi.org/10.3892/or.16.1.183
MLA
Ciotti, M., Giuliani, L., Ambrogi, V., Ronci, C., Benedetto, A., Mineo, T. C., Syrjänen, K., Favalli, C."Detection and expression of human papillomavirus oncogenes in non-small cell lung cancer". Oncology Reports 16.1 (2006): 183-189.
Chicago
Ciotti, M., Giuliani, L., Ambrogi, V., Ronci, C., Benedetto, A., Mineo, T. C., Syrjänen, K., Favalli, C."Detection and expression of human papillomavirus oncogenes in non-small cell lung cancer". Oncology Reports 16, no. 1 (2006): 183-189. https://doi.org/10.3892/or.16.1.183