Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer

  • Authors:
    • M. Raida
    • T. Weiss
    • C. Leo
    • D. Lenz
    • A. Tarnok
    • K. Ameri
    • A. L. Harris
    • M. Höckel
    • D. Niederwieser
  • View Affiliations

  • Published online on: September 1, 2006     https://doi.org/10.3892/or.16.3.597
  • Pages: 597-601
Metrics: Total Views: 0 (Spandidos Publications: | PMC Statistics: )
Total PDF Downloads: 0 (Spandidos Publications: | PMC Statistics: )


Abstract

Tumor hypoxia leads to adaptive responses in cancer cells, including an induction of vasculogenesis initiated by circulating endothelial progenitor cells (EPCs) and circulating endothelial cells (CECs). The aim of the present study was to correlate the number of EPCs and CECs with the oxygenation of cervical cancer. Blood concentrations of EPCs were detected by FACS analysis with antibodies for CD34 and vascular endothelial growth factor receptor 2 (VEGFR2). CECs were evaluated by double staining for 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine-labeled acetylated low density lipoprotein (Di-LDL) and lectin in a cell culture assay. Ten patients with cervical cancer were compared with ten healthy volunteers. Intratumoral oxygen tension was assessed polarographically with the computerized Eppendorf histography system. Analysis of CEC numbers revealed no difference between patients and controls. However, patients had lower concentrations of CD34-positive hematopoietic stem cells (HSCs) but a significantly higher fraction of EPCs related to the number of HSCs (1.09% versus 0.53%). This fraction was significantly inversely correlated to the median oxygen tension (r=−0.74, p=0.015). Our study shows for the first time a significant inverse correlation between the fraction of EPCs and intratumoral oxygen tension. We conclude that the fraction of EPCs should be further evaluated as a useful and convenient marker in the prediction of tumor tissue oxygenation.

Related Articles

Journal Cover

September 2006
Volume 16 Issue 3

Print ISSN: 1021-335X
Online ISSN:1791-2431

Sign up for eToc alerts

Recommend to Library

Copy and paste a formatted citation
x
Spandidos Publications style
Raida M, Weiss T, Leo C, Lenz D, Tarnok A, Ameri K, Harris AL, Höckel M and Niederwieser D: Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer. Oncol Rep 16: 597-601, 2006
APA
Raida, M., Weiss, T., Leo, C., Lenz, D., Tarnok, A., Ameri, K. ... Niederwieser, D. (2006). Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer. Oncology Reports, 16, 597-601. https://doi.org/10.3892/or.16.3.597
MLA
Raida, M., Weiss, T., Leo, C., Lenz, D., Tarnok, A., Ameri, K., Harris, A. L., Höckel, M., Niederwieser, D."Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer". Oncology Reports 16.3 (2006): 597-601.
Chicago
Raida, M., Weiss, T., Leo, C., Lenz, D., Tarnok, A., Ameri, K., Harris, A. L., Höckel, M., Niederwieser, D."Circulating endothelial progenitor cells are inversely correlated with the median oxygen tension in the tumor tissue of patients with cervical cancer". Oncology Reports 16, no. 3 (2006): 597-601. https://doi.org/10.3892/or.16.3.597