Inhibition of murine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18

  • Authors:
    • C. P. Chikkanna-Gowda
    • S. McNally
    • B. J. Sheahan
    • M. N. Fleeton
    • G. J. Atkins
  • View Affiliations

  • Published online on: October 1, 2006     https://doi.org/10.3892/or.16.4.713
  • Pages: 713-719
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Abstract

The enhanced Semliki Forest virus vector (SFV10-E), an RNA-based suicide expression vector system, expresses foreign genes at levels up to 10× higher than the original SFV10 vector. This vector has been used previously to express interleukin-12 for a tumour treatment study in a BALB/c murine model. Interleukin-18, an IFN-γ-inducing cytokine, plays a key role in the early induction of T helper1 (Th1) cell-mediated immune responses in addition to anti-angiogenic activity. In this study, the murine IL-18 gene along with an Ig-kappa leader sequence was cloned into the SFV10-E vector. The pSFV10-E-IL-18 construct was characterised in vitro for levels of expression and secretion, and the production of biologically active IL-18 was confirmed. An in vivo tumour treatment study using high titre rSFV10-E-IL-18 virus-like particles to treat subcutaneous K-BALB and CT26 tumours in BALB/c mice demonstrated therapeutic efficacy including the disappearance of tumour cells in a minority of treated animals. Tumour regression was associated with induction of avascular and suppurative necrosis.

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October 2006
Volume 16 Issue 4

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Chikkanna-Gowda CP, McNally S, Sheahan BJ, Fleeton MN and Atkins GJ: Inhibition of murine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18. Oncol Rep 16: 713-719, 2006
APA
Chikkanna-Gowda, C.P., McNally, S., Sheahan, B.J., Fleeton, M.N., & Atkins, G.J. (2006). Inhibition of murine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18. Oncology Reports, 16, 713-719. https://doi.org/10.3892/or.16.4.713
MLA
Chikkanna-Gowda, C. P., McNally, S., Sheahan, B. J., Fleeton, M. N., Atkins, G. J."Inhibition of murine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18". Oncology Reports 16.4 (2006): 713-719.
Chicago
Chikkanna-Gowda, C. P., McNally, S., Sheahan, B. J., Fleeton, M. N., Atkins, G. J."Inhibition of murine K-BALB and CT26 tumour growth using a Semliki Forest virus vector with enhanced expression of IL-18". Oncology Reports 16, no. 4 (2006): 713-719. https://doi.org/10.3892/or.16.4.713