Connexin 32 expression reduces malignant phenotype in human A549 adenocarcinoma cells: Implication of Src involvement

  • Authors:
    • Sachio Hada
    • Hiromi Sato
    • Nantiga Virgona
    • Hiromi Hagiwara
    • Terunobu Saito
    • Kazuyuki Suzuki
    • Ryuji Asano
    • Tomohiro Yano
  • View Affiliations

  • Published online on: November 1, 2006     https://doi.org/10.3892/or.16.5.1149
  • Pages: 1149-1154
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Abstract

Recent evidence suggests that a member of the gap junction protein family, connexin (Cx) 32, acts as a tumor suppressor gene against lung adenocarcinoma. However, the precise mechanism remains unclear. In this study, we tried to explore the mechanism for the Cx32-dependent tumor-suppressive effect in lung adenocarcinoma. To perform this study, we established a stable clone of the human lung adenocarcinoma cell line, A549 in which the Cx32 gene was expressed. Cx32 expression in A549 cells reduced anchorage-independent growth and development of tumors in a xenograft model. Additionally, Cx32 induced contact inhibition of growth and reduced invasive activity in A549 cells. The tumor-suppressive effects of Cx32 depended on the inhibition of Src activity. These events were confirmed by an Src inhibitor (PP1) and siRNA for Cx32. These results suggest that the Cx32-dependent tumor-suppressive effect in A549 cells is explained by the inhibition of Src activity.

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November 2006
Volume 16 Issue 5

Print ISSN: 1021-335X
Online ISSN:1791-2431

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Spandidos Publications style
Hada S, Sato H, Virgona N, Hagiwara H, Saito T, Suzuki K, Asano R and Yano T: Connexin 32 expression reduces malignant phenotype in human A549 adenocarcinoma cells: Implication of Src involvement. Oncol Rep 16: 1149-1154, 2006
APA
Hada, S., Sato, H., Virgona, N., Hagiwara, H., Saito, T., Suzuki, K. ... Yano, T. (2006). Connexin 32 expression reduces malignant phenotype in human A549 adenocarcinoma cells: Implication of Src involvement. Oncology Reports, 16, 1149-1154. https://doi.org/10.3892/or.16.5.1149
MLA
Hada, S., Sato, H., Virgona, N., Hagiwara, H., Saito, T., Suzuki, K., Asano, R., Yano, T."Connexin 32 expression reduces malignant phenotype in human A549 adenocarcinoma cells: Implication of Src involvement". Oncology Reports 16.5 (2006): 1149-1154.
Chicago
Hada, S., Sato, H., Virgona, N., Hagiwara, H., Saito, T., Suzuki, K., Asano, R., Yano, T."Connexin 32 expression reduces malignant phenotype in human A549 adenocarcinoma cells: Implication of Src involvement". Oncology Reports 16, no. 5 (2006): 1149-1154. https://doi.org/10.3892/or.16.5.1149